中国汉族儿童TIRAP基因单核苷酸多态性分析未显示其与结核病的易感相关

发布时间：2018-04-30 06:59

本文选题：TIRAP + 多态性　；参考：《首都医科大学学报》2015年04期

【摘要】：目的探讨中国汉族儿童TIRAP基因Tag SNP位点的多态性与结核病易感性相关关系,以明确TIRAP基因在儿童结核病发病中的作用。方法本课题组收集339名汉族儿童结核病患者,以同期查体的392名儿童作为对照,采用病例对照研究,应用高通量Mass ARRAY技术对于TIRAP基因的SNP位点进行基因分型研究。使用的是改进的盐析法进行基因组DNA的提取。结果 TIRAP基因的5个Tag SNPs(rs563011、rs8177400、rs1786704、rs8177375、rs8177352),除了1个SNP位点(rs563011)等位基因检出率95%及1个(rs8177400)AA基因型在人群中的分布为0,另外3个SNPs位点(rs1786704、rs8177375、rs8177352)的等位基因、基因型以及单体型在结核病组和对照组的分布差异均无统计学意义(P0.05)。结论 TIRAP基因多态性与中国汉族儿童结核病易感不存在相关性。
[Abstract]:Objective to investigate the relationship between the polymorphism of TIRAP gene Tag SNP locus and the susceptibility to tuberculosis in Chinese Han children, and to clarify the role of TIRAP gene in the pathogenesis of tuberculosis in children. Methods 339 children with tuberculosis in Han nationality were collected and 392 children were selected as controls. The high throughput Mass ARRAY technique was used to study the genotyping of SNP loci of TIRAP gene. An improved salting-out method was used to extract genomic DNA. Results the alleles of 5 Tag SNPs rs563011, rs8177400, rs1786704, rs8177375, rs8177352 and 1 rs8177400 AA allele of TIRAP gene were detected in 95% of the population except for one SNP locus, and the other three SNPs loci, rs1786704, rs817375rs8177352). There was no significant difference in the distribution of genotype and haplotype between tuberculosis group and control group (P 0.05). Conclusion there is no correlation between TIRAP gene polymorphism and susceptibility to tuberculosis in Chinese Han children.
【作者单位】：首都医科大学附属北京儿童医院北京市儿科研究所结核病研究室儿科学国家重点学科教育部儿科重大疾病研究重点实验室儿童呼吸道感染性疾病研究北京市重点实验室;河北省胸科医院结核科河北省医学重点发展学科;河北省儿童医院感染消化科;
【分类号】：R529.9

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