四种试验方法检测早期梅毒患者的结果分析

发布时间：2018-05-29 20:04

本文选题：早期梅毒 + 镀银染色　；参考：《中国艾滋病性病》2017年06期

【摘要】：目的了解镀银染色法、聚合酶链式反应(PCR)、梅毒甲苯胺红不加热血清试验(TRUST)和梅毒明胶颗粒凝集试验(TPPA)等4种方法,对检测早期梅毒患者的临床价值。方法 2012年1月至2016年7月,在广西部分地区的性病门诊收集疑似早期梅毒患者300例,分别采集组织液与血清标本。组织液标本采用镀银染色法检测梅毒螺旋体,PCR方法检测TP polA基因;血清标本用于TRUST检测非梅毒螺旋体抗体,TPPA检测梅毒螺旋体抗体。统计学资料采用SPSS 13.0软件进行分析。结果在215例确诊的早期梅毒患者中,梅毒PCR检测阳性标本210例,敏感性为97.7%;镀银染色法检测出105例,敏感性为48.8%;TRUST检测出196例,敏感性为91.2%;TPPA检测出208例,敏感性为96.7%。结论不同试验检测方法对早期梅毒患者的诊断有不同的临床应用价值,一期梅毒应当做病原学检查及梅毒血清学试验,二期梅毒应当以梅毒血清学试验为主,当出现结果异常时,应要求患者进行随访再检测以明确诊断。
[Abstract]:Objective to investigate the clinical value of silver staining, polymerase chain reaction (PCR), toluidine red unheated serum test (trust) and syphilis gelatin agglutination test (TPPA) in early syphilis patients. Methods from January 2012 to July 2016, 300 suspected early syphilis patients were collected from STD clinics in Guangxi. TP polA gene of Treponema pallidum was detected by silver staining in tissue solution and TRUST was used to detect Treponema pallidum antibody in serum. Statistical data were analyzed by SPSS 13.0 software. Results among 215 cases of early syphilis, 210 cases were positive for syphilis, the sensitivity was 97.7, the sensitivity was 48.8%, the sensitivity was 91.2%, the sensitivity was 96.7%. Conclusion different test methods have different clinical application value in the diagnosis of early syphilis. The primary syphilis should be tested by etiology and serological test of syphilis, and the serological test of syphilis should be given priority to in secondary syphilis. When abnormal results occur, patients should be asked to follow up and re-examine to make sure the diagnosis.
【作者单位】：广西壮族自治区皮肤病防治研究所;中国医学科学院皮肤病研究所;
【基金】：广西医疗卫生适宜技术研究与开发项目(S201539)~~
【分类号】：R759.11

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