海南省脑膜炎奈瑟菌多位点序列分型研究

发布时间：2018-09-19 20:33

【摘要】：目的了解海南省脑膜炎奈瑟菌(Nm)的分子分型特征。方法采用多位点序列分析(MLST)技术,对2005—2015年海南省流行性脑脊髓膜炎(ECM)病人、密切接触者和健康带菌者样本中分离到的16株Nm和1份脑脊液样本进行核酸序列分析。结果 17份样本共分为11种不同的Nm ST型别,分属于ST-4821、ST-11、ST-5、ST-175、ST-364克隆群和5个无序列群分类(UA),其中前3个克隆群为高致病性克隆群,分别占29.41%(5株)、17.65%(3株)、5.88%(1株)。ST-4821克隆群有ST-4821型和ST-3200型。ST-4821型来自3例C群ECM病例和1株不可分群密切接触者。ST-3200型来自2005年的C群健康带菌者。ST-11、ST-5克隆群分别只有W135群的ST-11型和不可分群的ST-7型,均来自健康带菌者。B群5株有5种ST,其中ST-11127型和ST-11134型为新发现的ST,2015年海口市首例B群病人是ST-7962型,与2010年分离自儋州市ECM密切接触者的B群ST-11127型同源,均为UA,其他不同基因群序列型相互间进化关系较远。结论海南省Nm分子型别具有基因多样性,Nm C群ST-4821型是优势序列群。健康人群中携带有高致病性克隆群菌株。
[Abstract]:Objective to investigate the molecular typing characteristics of Neisseria meningitidis (Nm) in Hainan Province. Methods the nucleic acid sequences of 16 strains of Nm and 1 sample of cerebrospinal fluid (CSF) isolated from (ECM) patients, close contacts and healthy carriers of epidemic meningitis in Hainan Province from 2005 to 2015 were analyzed by multilocus sequence analysis (MLST). Results the 17 samples were divided into 11 different Nm ST types, belonging to ST-4821,ST-11,ST-5,ST-175,ST-364 clone and 5 nonsequence-free clones. The first three clones were highly pathogenic clones. 5.88% (1 strain). ST-4821 clone group: ST-4821 type and ST-3200 type .ST-4821 type from three cases of group C ECM cases and one indistinguishable group of close contacts. ST-3200 from 2005 group C healthy carrier. ST-11ST-5 clone has only ST-11 type and W135 group. ST-7 type, which can not be divided into groups, There were 5 ST, types in all 5 strains from healthy carriers. Among them, ST-11127 type and ST-11134 type were newly discovered. The first group B patient in Haikou City in 2015 was ST-7962 type, which was homologous to group B ST-11127 type isolated from ECM close contact in Danzhou City in 2010. All of them were related to the evolution of other gene groups in UA,. Conclusion the Nm molecular type in Hainan Province is a dominant sequence group with gene diversity and Nm C ST-4821 type. High pathogenic clone strains were carried in healthy population.
【作者单位】：海南省疾病预防控制中心检验检测所微生物检验科;
【基金】：海南省自然科学基金(814339) 海南省卫计委资助项目(琼卫2013资助-057号) 国家科技重大专项课题(2013ZX10004-203-002-006)
【分类号】：R515.2

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