蛋白免疫印迹实验MP试剂快速法与隔夜孵育法的比对结果分析

发布时间：2018-10-09 14:37

【摘要】：目的为了减少艾滋病病毒(HIV)确证实验室"不确定"样本的例数,使感染早期病人得到及时诊断,晚期病人得到及时治疗。方法对2012-2013年HIV-1抗体筛查阳性血清样本,用蛋白免疫印迹试验(WB)MP试剂快速法进行确证检测,确证结果为"不确定"的样本,采用MP试剂隔夜孵育法与之进行了比对。结果 46例WB"阳性-不确定"样本中,22例"不确定-阴性"及4例"不确定-失访"样本,其隔夜孵育法与快速法相比,确证条带数均未增加,部分样本颜色有不同程度的加深。20例"不确定-阳性"样本,有10例条带数在原有的基础上有不同程度的增加,并完全满足国家阳性判断标准,无需随访就可以确证为"阳性"结果;另10例条带在原有的基础上均有加深,其中3例符合阳性判断标准。对于20例"不确定-阳性"样本,隔夜孵育法与快速法差异有显著的统计学意义(P0.05)。结论隔夜孵育法与快速法相比,反应时间较长,血清中的抗体与纤维膜上的抗原结合比较充分,一些早期或晚期感染病例在快速法中不显现的条带在隔夜孵育法得到了充分的展现。建议确证实验室如采用MP试剂快速法的,当结果为"阳性-不确定"的情况下,同时筛查结果为三种以上阳性者,应采用隔夜孵育法进行复测确证实验。
[Abstract]:Objective in order to reduce the number of "uncertain" samples in the laboratory of (HIV) confirmation of HIV, to make the early infected patients get timely diagnosis and the late patients to receive timely treatment. Methods the positive serum samples screened for HIV-1 antibody in 2012-2013 were confirmed by Western blot (WB) MP reagent method. The results were confirmed as "uncertain" samples. The results were compared with MP reagent overnight incubation method. Results of 46 WB "positive-uncertainty" samples, 22 were "uncertain negative" and 4 were "uncertain-lost" samples. The number of confirmed bands was not increased by overnight incubation method compared with the rapid method. The color of some samples deepened in varying degrees. 20 cases of "uncertain positive" samples, and 10 cases of bands increased in varying degrees on the original basis, and fully met the national criteria for positive judgment. The "positive" results could be confirmed without follow-up, and the bands of the other 10 cases were deepened on the original basis, among which 3 cases met the criteria of positive judgment. For 20 cases of "uncertain-positive" samples, there was significant difference between overnight incubation method and fast method (P0.05). Conclusion the reaction time of the overnight incubation method is longer than that of the fast method, and the antibody in the serum binds more fully to the antigens on the fibrous membrane. Some early or late infection cases do not appear in the rapid method in the overnight incubation method has been fully demonstrated. It is suggested that if the rapid method of MP reagent is used in the confirmation laboratory, if the result is "positive-uncertain" and the screening result is more than three kinds of positive, the overnight incubation method should be used to retest the confirmation experiment.
【作者单位】：上海浦东新区疾病预防控制中心;
【基金】：浦东新区卫生系统优秀青年医学人才培养项目(PWRQ2013-22)~~
【分类号】：R512.91;R446.6

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