基因芯片方法检测耐异烟肼结核分枝杆菌准确性的Meta分析

发布时间：2018-10-14 11:00

【摘要】：目的利用Meta分析方法系统评价基因芯片技术检测耐异烟肼(INH)结核分枝杆菌的准确性。方法计算机检索The Cochrane Library、SCI、PubMed、EMbase、WanFang Data、CNKI数据库,检索时限均为建库至2015年5月。由2位研究者根据纳入与排除标准筛选文献、提取资料,应用QUADAS条目工具评价纳入研究质量,采用Meta-DiSc 1.4软件对敏感性(SEN)、特异性(SPE)、阳性似然比(+LR)、阴性似然比(-LR)、诊断比值比(DOR)进行异质性检验和合并分析并绘制受试者工作特征(SROC)曲线、计算曲线下面积(AUC)。结果共纳入15篇符合要求文献,分析3 967株经传统药敏试验鉴定结核分枝杆菌菌株,其中耐INH菌株1 147株、敏感菌株2 820株。基因芯片技术检测耐INH结核分枝杆菌的SEN_(并发)为0.82[95%CI(0.79~0.84)]、SPE_(并发)为0.97[95%CI(0.97~0.98)]、+LR为21.81[95%CI(12.12~39.25)]、-LR为0.19[95%CI(0.15~0.24)]、DOR_(并发)为136.55[95%CI(70.66~263.89)],AUC为0.94。结论通过循证学方法系统评价基因芯片技术检测耐INH结核分枝杆菌结果表明,其具有较好的诊断价值;与传统药敏试验相比基因芯片技术操作简单、检测快速,对耐药基因检出率较高,但费用较贵,成本较高。
[Abstract]:Objective to evaluate the accuracy of gene chip technique for the detection of isoniazid resistant (INH) Mycobacterium tuberculosis by Meta. Methods The Cochrane Library,SCI,PubMed,EMbase,WanFang Data,CNKI database was searched by computer, and the retrieval time was from May 2015 to May 2015. According to the inclusion and exclusion criteria, two researchers sifted the literature, extracted the data, and used the QUADAS entry tool to evaluate the quality of the inclusion study. The Meta-DiSc 1.4 software was used to test the heterogeneity of the sensitive (SEN), specific (SPE), positive likelihood ratio to the (LR), negative likelihood ratio (- LR), diagnostic ratio was higher than (DOR) and to draw the (SROC) curve of the operating characteristics of the subjects. The area under the curve was calculated by (AUC). Results A total of 15 documents were included, and 3 967 strains of Mycobacterium tuberculosis were identified by traditional drug sensitivity test, including 1 147 strains resistant to INH and 2 820 strains of sensitive strains. SEN_ (concurrent) of Mycobacterium tuberculosis resistant to INH was 0.82 [95%CI (0.790.84)], SPE_ (concurrent) was 0.97 [95%CI (0.970.98)], LR was 21.81 [95%CI (12.1239.25), -LR was 0.19 [95%CI (0.150.24)], DOR_ (complicated) was 136.55 [95%CI (70.66) 263.89), AUC was 0.9448. Conclusion the results of systematically evaluating the gene chip technique for the detection of Mycobacterium tuberculosis resistant to INH by Evidence-based method show that it has a good diagnostic value, and compared with the traditional drug sensitivity test, the gene chip technique is simple and rapid. The detection rate of drug resistance gene is higher, but the cost is higher and the cost is higher.
【作者单位】：四川大学生命科学学院;成都市第七人民医院检验科;
【分类号】：R440;R52

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