2013-2014年北京市怀柔区散发腹泻患者的腺病毒感染调查

发布时间：2018-10-22 12:27

【摘要】：目的了解北京怀柔地区腹泻患者腺病毒感染情况及其分子特征。方法采集怀柔区2013年4月至2014年3月120例腹泻患者粪便标本,用聚合酶链反应(PCR)方法检测腺病毒hexon区域,然后对PCR阳性扩增产物进行测序。采用Mega软件对序列进行比对并构建进化树。结果 120例标本中腺病毒阳性24例,检出率为20.0%;其中7月龄到2岁婴幼儿患者检出率最高,为26.7%(16/60);2个感染高峰分别为6-8月和10-11月,其余月份有腺病毒感染的散发。对其中18例腺病毒hexon区域PCR阳性产物测序和系统进化分析显示,13例为腺病毒F组41型(72.2%,13/18),2例为F组40型(11.1%,2/18),2例为C组的Ad2和Ad6(11.1%,2/18),A组31型1例。结论腺病毒是怀柔地区2013-2014年病毒性腹泻的重要病原之一,F组41型为优势流行株,同时有其他型别的散发。
[Abstract]:Objective to investigate the status and molecular characteristics of adenovirus infection in diarrhea patients in Huairou district of Beijing. Methods the feces of 120 diarrhea patients from April 2013 to March 2014 were collected in Huairou District. The adenovirus hexon region was detected by polymerase chain reaction (PCR) and the PCR positive amplification products were sequenced. The sequence alignment was carried out by Mega software and the evolutionary tree was constructed. Results the positive rate of adenovirus was 20.0 in 120 specimens, the highest rate was 26.7% (16 / 60) in infants aged from 7 months to 2 years old, and the peak of two infection peaks were June to August and October to November respectively, and there were sporadic adenovirus infection in other months. The sequencing and phylogenetic analysis of PCR positive products of adenovirus hexon region in 18 cases showed that 13 cases were adenovirus group F 41 (72.2% 18), 2 cases were group F 40 (11.1% / 18), 2 cases were Ad2 and Ad6 (11.1%), A), 1 case was group 31. Conclusion adenovirus is one of the most important pathogens of viral diarrhea in Huairou area from 2013 to 2014. Group F 41 is the dominant epidemic strain with other types of distribution.
【作者单位】：北京市怀柔区疾病预防控制中心;北京市疾病预防控制中心;
【基金】：北京市自然科学基金资助项目“北京市食源性胃肠炎暴发和聚集性病例中诺如病毒的分子流行病学研究”(7132045)
【分类号】：R512.5;R440

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