TEA敏感型钾电流在gp120引起的海马神经元损伤中的作用

发布时间：2018-11-28 13:55

【摘要】：目的:探讨gp120对TEA敏感型钾电流的作用以及该电流在gp120引起神经元损伤中的作用。方法 :分离怀孕18d SD大鼠胎鼠的海马神经元作为实验对象,分为对照组和gp120处理组,利用全细胞膜片钳的方法记录外向延迟钾电流的变化,通过MTT和TUNEL的方法分别观察gp120处理后神经元的活力和凋亡情况。Western blot观察Kv2.1钾通道的蛋白表达变化以及在TEA敏感型钾电流中的贡献度。结果:gp120能引起外向钾电流明显增大,具有浓度依赖性,其中TEA敏感型成分参与了外向钾电流的增大。此外,MTT和TUNEL实验发现,gp120能引起神经元活力的下降和凋亡,而钾通道抑制剂TEA能缓解由gp120引起的细胞损伤。进一步实验表明,gp120可上调TEA敏感型成分中Kv2.1钾通道的表达,且其特异性抑制剂Gx TX-1E能显著抑制神经元中TEA敏感型钾电流成分。结论:TEA敏感型钾电流参与了gp120引起的神经元损伤过程,其重要组成成分Kv2.1通道蛋白上调,提示该通道蛋白可能参与了gp120引起的细胞损伤。
[Abstract]:Aim: to investigate the effect of gp120 on TEA sensitive potassium current and its role in neuronal injury induced by gp120. Methods: the hippocampal neurons of 18 day pregnant SD rats were divided into two groups: control group and gp120 treatment group. The changes of outward delayed potassium currents were recorded by whole-cell patch clamp method. MTT and TUNEL were used to observe the neuronal activity and apoptosis after gp120 treatment. Western blot was used to observe the protein expression of Kv2.1 potassium channel and its contribution to TEA sensitive potassium current. Results: gp120 increased the outward potassium current in a dose-dependent manner, and the TEA sensitive component was involved in the increase of outward potassium current. In addition, MTT and TUNEL experiments showed that gp120 could induce the decrease of neuronal activity and apoptosis, while TEA, a potassium channel inhibitor, could attenuate the cell damage induced by gp120. Further experiments showed that gp120 could up-regulate the expression of Kv2.1 potassium channel in TEA sensitive components, and its specific inhibitor, Gx TX-1E, could significantly inhibit the TEA sensitive potassium current in neurons. Conclusion: TEA sensitive potassium current is involved in the process of neuronal injury induced by gp120, and its important component, Kv2.1 channel protein, is up-regulated, suggesting that this channel protein may be involved in the cell damage induced by gp120.
【作者单位】：南京医科大学公共卫生学院;南京医科大学第一附属医院急诊中心;南京医科大学公共卫生学院教育部重点实验室;
【基金】：国家自然科学基金面上项目(81072329);国家自然科学基金青年基金(81202230) 南京医科大学科技发展基金(2011NJMU275) 江苏省高校优势学科建设工程资助项目(CX09B-264Z) 临床医学科技专项(SBL2014020070)
【分类号】：R512.91

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