广州与佛山地区2014年登革病毒基因型特征研究
发布时间：2019-03-05 14:27
【摘要】：目的对2014年广州与佛山地区登革病毒C-prM区进行基因测序,从分子水平与相关国际流行株进行同源性比较,构建系统发育树,追踪其可能输入来源。方法收集本院2014年9月-11月447例登革热疑似患者急性期血清,随机选取其中广州与佛山地区不同区域的24例标本,提取病毒RNA,RT-PCR法扩增C-prM基因,并将其克隆到p MD19T载体,测序并利用生物信息学软件进行分析。结果成功测序登革病毒11株,BLAST分析表明均为登革1型病毒(DENV-1)。系统发育树结果显示GZ2014-01等序列与2010年印度德里流行株同源性高(相似度为99.21%),GZ2014-02等序列与2013年广州流行株相近(相似度为97.64%~98.82%)。广州株GZ2014-01等和佛山株GZ2014-23属于基因型Ⅴ,而广州株GZ2014-02等和佛山株GZ2014-20属于基因型Ⅰ。结论 GZ2014-01等株可能来源于印度德里,而GZ2014-02等株可能来源于中国广州。登革病毒多基因型流行可能是造成2014年登革热大暴发的原因之一。
[Abstract]:Aim to sequence the C-prM region of dengue virus in Guangzhou and Foshan in 2014 and compare it with the related international strains at molecular level in order to construct phylogenetic tree and trace its possible input source. Methods Serum samples were collected from 447 suspected dengue fever patients in our hospital from September to November, 2014. 24 samples from different regions of Guangzhou and Foshan were randomly selected and the C-prM gene was amplified by viral RNA,RT-PCR. It was cloned into p-MD19T vector, sequenced and analyzed by bioinformatics software. Results 11 strains of dengue virus were sequenced successfully. BLAST analysis showed that all strains were dengue type 1 virus (DENV-1). Phylogenetic tree analysis showed that GZ2014-01 et al had high homology with India Delhi epidemic strain in 2010 (similarity was 99.21%), and GZ2014-02 et al sequence was similar to Guangzhou epidemic strain in 2013 (97.64% and 98.82% similarity). Guangzhou strain GZ2014-01 et al and Foshan strain GZ2014-23 belong to genotype 鈪，
本文编号：2435001
[Abstract]:Aim to sequence the C-prM region of dengue virus in Guangzhou and Foshan in 2014 and compare it with the related international strains at molecular level in order to construct phylogenetic tree and trace its possible input source. Methods Serum samples were collected from 447 suspected dengue fever patients in our hospital from September to November, 2014. 24 samples from different regions of Guangzhou and Foshan were randomly selected and the C-prM gene was amplified by viral RNA,RT-PCR. It was cloned into p-MD19T vector, sequenced and analyzed by bioinformatics software. Results 11 strains of dengue virus were sequenced successfully. BLAST analysis showed that all strains were dengue type 1 virus (DENV-1). Phylogenetic tree analysis showed that GZ2014-01 et al had high homology with India Delhi epidemic strain in 2010 (similarity was 99.21%), and GZ2014-02 et al sequence was similar to Guangzhou epidemic strain in 2013 (97.64% and 98.82% similarity). Guangzhou strain GZ2014-01 et al and Foshan strain GZ2014-23 belong to genotype 鈪，
本文编号：2435001
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