利福平对结核分枝杆菌在不同植入材料界面黏附力及生物膜形成的影响
发布时间:2019-04-10 16:01
【摘要】:目的通过体外实验研究利福平对结核分枝杆菌(MTB)在不同植入材料界面黏附力及生物膜形成的影响。方法随机选取钴铬钼合金、钛合金、聚乙烯样品各两块分别置入3支离心管中,加入8 m L Middlebrook7H9液体培养基,再接种初始浓度为1010CFU/m L的MTB悬液2 m L混匀,恒温(37℃)下培养30 d,从每支离心管中,随机取出一块材料,经固定、干燥、喷金后,应用扫描电子显微镜观察,比较MTB对不同植入材料的黏附力,同时观察界面生物膜形成情况。然后每支离心管中加入2 m L浓度为1μg/m L的利福平溶液,同样条件下继续培养30 d。取出材料块,经固定、干燥、喷金后,应用扫描电子显微镜观察,了解利福平对MTB黏附力的影响及对MTB生物膜的破坏情况。结果 MTB对不同植入材料界面的黏附力由强到弱依次是聚乙烯、钴铬钼、钛合金;聚乙烯界面可见生物膜形成,钴铬钼合金、钛合金界面均未见生物膜形成;利福平干预后,植入物界面黏附的菌落数显著减少,聚乙烯界面生物膜呈不同程度破坏。结论利福平能抑制MTB对不同植入材料界面的黏附及生物膜形成。
[Abstract]:Aim to study the effect of rifampicin on the adhesion force and biofilm formation of Mycobacterium tuberculosis (MTB) at the interface of different implanted materials in vitro. Methods Cobalt-chromium-molybdenum alloy and titanium alloy were randomly selected. Two samples of polyethylene were placed in three centrifuges respectively, then added 8 mL Middlebrook7H9 liquid medium, then inoculated with MTB suspension with initial concentration of 1010CFU/m / L for 2 mL mixing. After being cultured at 37 鈩,
本文编号:2455933
[Abstract]:Aim to study the effect of rifampicin on the adhesion force and biofilm formation of Mycobacterium tuberculosis (MTB) at the interface of different implanted materials in vitro. Methods Cobalt-chromium-molybdenum alloy and titanium alloy were randomly selected. Two samples of polyethylene were placed in three centrifuges respectively, then added 8 mL Middlebrook7H9 liquid medium, then inoculated with MTB suspension with initial concentration of 1010CFU/m / L for 2 mL mixing. After being cultured at 37 鈩,
本文编号:2455933
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