齐多夫定和单克隆抗体联合应用去除疫苗毒种中REV的污染

发布时间：2018-01-31 00:10

本文关键词： 禽网状内皮组织增生症病毒(REV) 齐多夫定(AZT) 单克隆抗体弱毒疫苗污染　出处：《病毒学报》2017年04期 　论文类型：期刊论文

【摘要】：禽网状内皮组织增生症病毒(REV)在禽弱毒疫苗中的污染被认为是REV感染的途径之一,去除疫苗毒种中REV的污染是生产合格疫苗的前提,此前我们研究证实了某IBDV疫苗毒种存在REV的污染,本研究试图通过核苷类逆转录酶抑制剂类药物齐多夫定(Azidothymidine,AZT)和针对REV的单克隆抗体(McAb)单独或联合应用来去除疫苗毒种中REV的污染。将污染REV的IBDV疫苗毒种接种DF-1细胞后,分别经5μg/mL的AZT,5%的McAb或两者联合应用干预后,毒种中污染的REV复制受到了明显的干扰,特别是经过AZT与McAb联合应用两次干预后,检测REV为完全阴性,毒种经检验合格。本研究通过AZT与McAb联合应用成功净化了IBDV疫苗毒种中REV的污染,为解决类似问题提供了新的思路。
[Abstract]:The contamination of reticuloendotheliosis virus (Rev) in avian attenuated virus vaccine is considered to be one of the routes of REV infection, and the removal of REV contamination from vaccine strains is the prerequisite for the production of qualified vaccine. Previous studies have confirmed the presence of REV contamination in a IBDV vaccine strain. This study attempted to use azidothymidine as a nucleoside reverse transcriptase inhibitor. AZTand monoclonal antibody against REV should be used either alone or in combination to remove the contamination of REV in the vaccine strain. After inoculating the IBDV vaccine strain contaminated with REV into DF-1 cells. After 5 渭 g / mL of AZT5% McAb or a combination of AZT5% or the combination of AZT5%, the replication of contaminated REV in the virus species was obviously interfered. In particular, after two interventions of AZT and McAb, REV was completely negative. The AZT and McAb were used to purify the REV contamination in the IBDV vaccine successfully, which provided a new way to solve the similar problems.
【作者单位】：山东农业大学动物医学院;
【基金】：国家自然科学基金(项目号:31402226),题目:禽白血病病毒在药物选择压下的基因变异和准种演变~~
【分类号】：S852.4
【正文快照】： 禽网状内皮组织增生症(Reticuloendothelio-sis,RE)是由禽网状内皮组织增生症病毒(Reticu-loendotheliosis virus,REV)引起的禽类的一种重要的致肿瘤性疾病和免疫抑制性疾病,可以感染鸡、火鸡、鸭、鹅、鹌鹑和野鸡等。除垂直传播外,使用了被REV污染的弱毒疫苗被认为是导致REV

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