Wnt2在奶牛胎盘发育过程中的表达与定位

发布时间：2018-02-10 00:08

本文关键词： 兽医学 Wnt 胎盘发育奶牛　出处：《中国科技论文》2017年18期 　论文类型：期刊论文

【摘要】：胎盘发育异常是体细胞克隆失败的主要原因之一,但其机制知之甚少,而Wnt信号通路广泛参与细胞命运决定、胎儿发育和胎盘形成等生命过程。采用qRT-PCR和原位杂交等方法检测Wnt2在奶牛胎盘中的表达与定位,发现Wnt2主要富集在子叶中并定位于子叶滋养层细胞上。结果表明,Wnt2在奶牛胎盘发育过程中发挥着重要的调节功能。为解决奶牛胎盘发育异常的问题找到新的分子作用靶点。
[Abstract]:Placental dysplasia is one of the main reasons for the failure of somatic cell cloning, but its mechanism is poorly understood, and the Wnt signaling pathway is widely involved in cell fate. QRT-PCR and in situ hybridization were used to detect the expression and localization of Wnt2 in bovine placenta. It was found that Wnt2 was mainly concentrated in cotyledons and located on the trophoblast cells of cotyledons. The results showed that WNT2 played an important regulatory role in the development of dairy cattle placenta, and a new molecular target was found to solve the problem of abnormal placenta development in dairy cows.
【作者单位】：黑龙江八一农垦大学动物科技学院;
【基金】：高等学校博士学科点专项科研基金资助项目(20132305120003) 校学成、引进人才科研启动计划资助项目(2031011032/011032-科研启动)
【分类号】：S823

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