内质网途径在硫酸黏菌素致PC12细胞凋亡中的作用

发布时间：2018-02-10 00:33

本文关键词： 硫酸黏菌素 PC细胞细胞凋亡内质网途径　出处：《现代畜牧兽医》2016年12期 　论文类型：期刊论文

【摘要】：为了探究内质网途径在硫酸黏菌素诱导PC12细胞凋亡中的作用。取对数生长期PC12细胞,用含0、62.5、125、250μg/m L硫酸黏菌素的DMEM培养液作用24 h,透射电镜观察PC12细胞凋亡,Western blot法检测Gn RP78、caspase-12、Caspase-3蛋白表达量,Fluo-3/AM检测细胞内[Ca~(2+)]i浓度变化,钙测定试剂盒检测细胞外[Ca~(2+)]i浓度变化。与对照组相比,125、250μg/m L硫酸黏菌素剂量组PC12细胞出现典型凋亡现象,使PC12细胞内[Ca~(2+)]i浓度,Gn RP78、caspase-12、Caspase-3蛋白表达量显著升高(P0.01);细胞外[Ca~(2+)]i浓度显著降低(P0.01),具有剂量-效应关系。说明通过内质网途径介导的PC12细胞凋亡可引发硫酸黏菌素的神经毒性。
[Abstract]:In order to explore the role of endoplasmic reticulum pathway in apoptosis of PC12 cells induced by myxin sulfate, the logarithmic phase of PC12 cells was obtained. After exposed to DMEM medium containing 250 渭 g / mL myxin sulfate for 24 h, the apoptosis of PC12 cells was observed by transmission electron microscope (TEM). The expression of Caspase-12Caspase-3 protein in PC12 cells was detected by Western blot method. Fluo-3 / AM was used to detect the concentration of [Ca~(2] I in cells. The concentration of extracellular [Ca~(2] I was detected by calcium assay kit. Compared with the control group, the apoptosis of PC12 cells in the 250 渭 g / mL mucin sulfate dose group was typical. The concentration of [Ca~(2] I in PC12 cells and the expression of Gn RP78Caspase-12 Caspase-3 protein were significantly increased in PC12 cells, and the concentration of extracellular [Ca~(2] I was significantly decreased in a dose-effect relationship. It was suggested that apoptosis of PC12 cells mediated by endoplasmic reticulum pathway could induce neurotoxicity of myxin sulfate.
【作者单位】：锦州医科大学;东北农业大学;辽宁省兴城市药王动物卫生监督所;辽宁省锦州市义县留龙沟动物卫生监督所;
【基金】：辽宁省大学生创新创业项目(项目编号:201410160049) 辽宁省科学技术基金自然科学基金项目(项目编号:2014022051)
【分类号】：S852.6

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