肉鸡腹水综合征的gallus转录组学分析及丹参酮ⅡA对其防治的研究

发布时间：2018-03-03 00:15

本文选题：肉鸡　切入点：腹水综合征　出处：《江西农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本实验对患有腹水综合征的肉鸡肺动脉进行了gallus转录组生物信息分析,并研究了丹参酮IIA对其的防治效果。实验一:将75羽的15日龄健康AA肉鸡随机分成对照组(20羽)和造病组(55羽),造病组喂给含0.3%Nacl的高盐水,对照组正常饲喂,在出现明显临床症状后颈静脉放血致死,采集各组织器官用于组织切片,RV/TV0.3的鸡肺动脉用于RNA-seq检测。实验二:将160羽的15日龄健康AA肉鸡随后随机分成对照组、预防组、治疗组、造病组各40羽。预防、治疗和造病组喂给含0.3%Nacl的高盐水,预防组喂给含0.02%的丹参酮IIA饲料,其它饲喂条件不变,治疗组从第29日龄喂给含0.04%的丹参酮IIA饲料。第28、35、42和49日龄每组选取10羽,称重,采血,剖杀,计算右心室/全心室比值及测定血液相关生化指标,分析丹参酮IIA对肉鸡腹水综合征防治效果。结果表明:(一)造病组和对照组相比,腹水综合征鸡生长缓慢并伴有组织器官的病变,肺动脉RNA-seq检测显示腹水鸡和正常鸡有895个差异表达基因,腹水综合征可导致Ribosome,Translation等1990个GO terms和Ribosome,Cytokine-cytokine receptor interaction等122个代谢通路发生变化。(二):与造病组相比,RV/TV值在0.22-0.25区间,其他三组鸡的数量显著较低;造病组鸡体重第35和42日龄显著低于对照和预防组(P0.05),第49日龄极显著低于其余三组(P0.01);与对照组相比,造病组血浆总蛋白、白蛋白、球蛋白、总胆红素、碱性磷酸酶和甘油三酯浓度均显著升高(P0.01或P0.05),治疗组在第28日龄时显著升高(P0.05),预防组无显著差异。造病组血浆尿素氮、肌酐和尿酸分别显著升高(P0.01或P0.05),治疗组和预防组大多无显著差异(P0.05)。造病组血浆钙离子、磷离子浓度、离子浓度、钠离子、钾离子及镁离子浓度均显著升高(P0.01或P0.05),预防组和治疗组钙离子、钠离子和磷离子浓度,治疗组血浆钾离子、镁离子和氯离子也显著升高(P0.01或P0.05)在第28日龄,第35、49日龄,治疗组和预防组个离子均无显著差异。本实验从基因和代谢通路等方面揭示了腹水综合征对肉鸡的发病机制。发现RV/TV值在0.22-0.25之间的肉鸡处于亚健康状态,并证明丹参酮IIA可有效防治肉鸡腹水综合征的发生,为丹参酮IIA防治肉鸡腹水综合征的临床应用提供了一定的理论基础。
[Abstract]:The gallus transcriptional bioinformatics of pulmonary artery in broilers with ascites syndrome was studied. The control effect of tanshinone IIA was studied. Experiment 1: 75 healthy 15 day-old AA broilers were randomly divided into control group (n = 20) and disease-making group (n = 55). The disease-making group was fed with 0.3Nacl high salt water, while the control group was fed with normal saline. After obvious clinical symptoms, the jugular vein bled to death. The pulmonary arteries of each tissue and organ were collected for the detection of RNA-seq with RV / TV0.3. Experiment 2: 160 healthy 15 day-old AA broilers were randomly divided into control group, prevention group and treatment group. The prophylaxis, treatment and disease group were fed with high salt water containing 0.3% NaCl, while the prevention group was fed with 0.02% tanshinone IIA feed, while the other groups were fed the same conditions. The treatment group was fed with tanshinone IIA feed containing 0.04% at the 29th day. 10 of them were selected for weighing, collecting blood, dissecting, calculating the ratio of right ventricle / whole ventricle and measuring the biochemical indexes of blood. The prevention and treatment effect of tanshinone IIA on ascites syndrome in broilers was analyzed. The results showed that the chickens with ascites syndrome grew slowly and had pathological changes of tissues and organs compared with the control group. Pulmonary artery RNA-seq test showed that there were 895 differentially expressed genes in ascites chickens and normal chickens. Ascites syndrome could cause changes in 122 metabolic pathways such as Rio terms and Ribosometrium Cytokine-cytokine receptor interaction. The number of the other three groups was significantly lower, the body weight of the diseased group was significantly lower than that of the control and prevention groups at the 35th and 42nd day, and the 49th day was significantly lower than that of the other three groups, and the plasma total protein, albumin, globulin in the diseased group were significantly lower than those in the control group. The concentrations of total bilirubin, alkaline phosphatase and triglyceride were significantly increased (P 0.01 or P 0.05), and those of treatment group were significantly higher than those of control group at the 28th day, but there was no significant difference between the prevention group and the control group, but there was no significant difference in plasma urea nitrogen between the two groups. Creatinine and uric acid increased P0.01 or P0.05, respectively. There was no significant difference between the treatment group and the prevention group. The plasma calcium ion, phosphorus ion concentration, ion concentration, sodium ion in the diseased group were significantly higher than those in the control group, and there was no significant difference between the treatment group and the prevention group. The concentrations of calcium, sodium and phosphorus ions in the prevention group and the treatment group were significantly higher than those in the control group. The plasma potassium ion, magnesium ion and chloride ion in the treatment group also increased significantly (P 0.01 or P 0.05) at the 28th and 3549 days of age. This study revealed the pathogenesis of ascites syndrome in broilers from the aspects of genes and metabolic pathways. It was found that broilers with RV/TV between 0.22 and 0.25 were in a sub-healthy state. It was proved that tanshinone IIA could effectively prevent and treat ascites syndrome in broilers, which provided a theoretical basis for the clinical application of tanshinone IIA in the prevention and treatment of ascites syndrome in broilers.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31

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