一株赛鸽源鸽圆环病毒全基因克隆与序列分析

发布时间：2018-03-04 11:01

本文选题：鸽圆环病毒(PiCV)　切入点：赛鸽　出处：《病毒学报》2017年05期 　论文类型：期刊论文

【摘要】：鸽圆环病毒(Pigeon circovirus,PiCV)是圆环病毒科的一个成员,是一种免疫抑制性病原。为了研究PiCV中国流行毒株的基因特征和遗传进化关系,本研究应用PCR方法对江苏省某赛鸽公棚的22份样品进行了PiCV检测,结果有6份样品为阳性。采用重叠PCR技术得到一株鸽圆环病毒全基因序列,命名为JS15-1(GenBank登录号:KX431143)。基因序列分析表明,JS15-1全长为2 034bp,包含3个主要开放阅读框。核苷酸同源性比较结果显示JS15-1与GenBank上登录的其他鸽圆环病毒分离株的全基因核苷酸同源性为85.3%~97.1%,与比利时分离的Bel20株同源性最高。不同鸽圆环病毒Rep基因同源性为92.1%~96.6%,Cap基因同源性为72.2%~99.4%。全基因遗传进化树显示JS15-1与之前的中国分离株处于不同的分支,而与Bel20的亲缘关系最近,Rep基因进化树显示JS15-1与Bel20、PiCV/Japan/2010、Ita4B位于同一分支,Cap基因进化树显示JS15-1与Bel20处于同一分支,亲缘关系最近。氨基酸序列比较发现,JS15-1与Bel20在Rep蛋白存在6个氨基酸残基的差异,而Cap蛋白存在1个氨基酸残基的差异和1个氨基酸残基的插入。研究结果表明,JS15-1极可能来源于比利时,而且发生了变异。
[Abstract]:Pigeon circovirus, a member of the family circoviridae, is an immunosuppressive pathogen. In order to study the genetic characteristics and genetic evolution of PiCV strains in China, In this study, PCR method was used to detect 22 samples from a racing pigeon shed in Jiangsu Province, and 6 samples were positive. A complete gene sequence of pigeon circovirus was obtained by overlapping PCR technique. It was named JS15-1(GenBank accession number KX4311433.The gene sequence analysis showed that the full length of JS15-1 was 2 034bp. it contained three main open reading frames. The nucleotide homology analysis showed that JS15-1 had the whole gene nucleotides with other pigeon circovirus isolates registered on GenBank. The homology of Rep gene of different pigeon circovirus was 92.1% and 92.2% 99.4% respectively. The phylogenetic tree showed that JS15-1 was in a different branch from the previous Chinese isolate. The closest phylogenetic relationship with Bel20 was that JS15-1 and Bel20 PiCVR / Japan / 2010 Ita4B were located in the same branch, and that JS15-1 and Bel20 were in the same branch. The amino acid sequence analysis showed that there were 6 amino acid residues in Rep protein between JS 15-1 and Bel20. However, the difference of one amino acid residue and the insertion of one amino acid residue in Cap protein showed that JJS15-1 probably originated from Belgium and had a variation.
【作者单位】：南京农业大学动物医学院;南京千里光生物技术有限公司;
【基金】：高校产学研合作创新项目(项目号:NAU201504-01),题目:赛鸽疾病诊断的研究与防治~~
【分类号】：S852.65

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