体外产气法评价辣木叶与玉米的组合效应

发布时间：2018-03-07 21:21

本文选题：辣木叶　切入点：玉米　出处：《中国畜牧杂志》2017年03期 　论文类型：期刊论文

【摘要】：本试验旨在以苜蓿为对照研究辣木叶与玉米的组合效应。辣木叶/苜蓿与玉米分别按100:0、80:20、60:40、40:60、20:80、0:100的比例进行组合,采用体外发酵法研究不同组合间的体外发酵参数及其组合效应值的变化。结果表明:随着玉米比例的升高,两组培养液pH、氨态氮(NH_3-N)浓度和乙丙比均呈下降趋势,而乙酸、丙酸、丁酸、总挥发性脂肪酸(TVFA)、48h产气量(48 h GP)和潜在产气量逐渐升高;辣木叶组和苜蓿组的pH、48h GP和丁酸无差异(P0.05),TVFA、乙酸、丙酸、产气速率和有机物消化率(OMD)极显著高于苜蓿组(P0.01);辣木叶组的48 h GP和微生物蛋白(MCP)组合效应值以及综合组合效应值(AEs)与苜蓿组无差异(P0.05),TVFA、乙酸和NH_3-N的组合效应值显著高于苜蓿组(P0.05);以48 h GP、NH_3-N、MCP、TVFA浓度和OMD的组合效应值以及综合组合效应值AEs为主要衡量指标,辣木叶与玉米的组合比例为60:40时,正组合效应达到最大。从本试验结果可以得知,辣木叶是一种与苜蓿营养价值相当的粗饲料资源,辣木叶与玉米比例为60:40时的饲料利用效率最高。
[Abstract]:The purpose of this experiment was to study the combination effect of spicy wood leaf and corn with alfalfa as a control. The ratio of spicy leaf / alfalfa and corn was 100: 0: 80: 20: 40: 40: 60: 60: 20: 20: 80: 100, respectively. In vitro fermentation method was used to study the changes of fermentation parameters and combination effects between different combinations. The results showed that the pH, NH _ 3-N) concentration and ethylene-propyl ratio of the two groups of culture medium decreased with the increase of corn proportion, but acetic acid and propionic acid decreased. Butyric acid, total volatile fatty acid (TVFA), total volatile fatty acid (TVFA) and potential gas production (GPP) and potential gas production increased gradually in 48h, pH value of GP and butyric acid were not different (P0.05TV-FAA, acetic acid, propionic acid), the pH value of GP and butyric acid were not different between pungent leaf group and alfalfa group. The gas production rate and organic matter digestibility were significantly higher than that of alfalfa group P0.01.The combined effect value of GP and microorganism protein (NH_3-N) and synthetic combination effect value (AEs) and alfalfa group had no difference (P0.05), and the combined effect value of acetic acid and NH_3-N was obvious. The concentration of NH _ 3-N MCP-TVFA, the combined effect value of OMD and the comprehensive combination effect value of AEs were taken as the main indexes, and the results were as follows: (1) the concentration of NH _ 3-N MCPTVFA in alfalfa group was higher than that of alfalfa group (P 0.05). The positive combination effect reached the maximum when the ratio of chilli leaf and corn was 60: 40. From the results of this experiment, we can know that the chilli leaf is a kind of forage resource which has the same nutritional value as alfalfa. The feed utilization efficiency was the highest when the ratio of chilli leaf to corn was 60: 40.
【作者单位】：浙江大学动物科学学院;浙江大学创新技术研究院;
【基金】：资助项目:辣木枝、叶用于家畜饲料的应用技术研发(15-518004-005)
【分类号】：S816

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