奶牛排卵延迟的诊断及其治疗的研究

发布时间：2018-03-07 22:12

本文选题：奶牛　切入点：排卵延迟　出处：《石河子大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:奶牛排卵延迟是规模化奶牛场的四大卵巢疾病之一。本研究立足于生产实践中的实际问题:奶牛场排卵延迟诊断错误、不准确、治疗没有针对性,以及没有配种后保胎等共性问题,深入系统的对奶牛发情到排卵和排卵直至黄体形成期间的激素、部分细胞因子的变化规律进行了检测分析,以期为建立奶牛排卵延迟的诊断和配种后保胎提供依据。在此基础上,对排卵延迟奶牛进行了优化方案的治疗。以上最终目的是为了建立奶牛排卵延迟诊断程序、方法和优化治疗和保胎方案,提高受胎率,从而减少奶牛的经济损失。方法:从西部牧业中心奶牛场选取排卵延迟奶牛12头,临床健康正常发情排卵奶牛20头,从发情到配种后15天,连续跟踪采血,应用ELISA方法检测采集血清中的P4、E2、LH、FSH和IGF-I浓度,其中排卵延迟组又分为两组,分别为输精时注射LHRH-A3(定为排卵延迟1组)和输精时不注射LHRH-A3(定为排卵延迟2组),分析其激素变化规律。同时应用50s型Tringa Vet便携式线阵B超仪在配种后7-15天进行跟踪扫查(隔2天1次),用B超仪内置的电子尺测量了黄体周长、黄体面积、黄体体积以及黄体直径,统计分析这些黄体指标的变化规律。依据以上研究,制定了奶牛排卵延迟的诊断方法和保胎方案,然后在奶牛场对常规激素治疗方案(LHRH-A3)、中药治疗组(理囊散)和优化治疗方案选择了60头排卵延迟奶牛进行了临床疗效观察。结果:第2天上午排卵延迟1组(注射LHRH-A3)血清LH浓度低于正常组奶牛且差异显著(P0.05),其它各个阶段差异均不显著(P0.05);排卵延迟1组(注射LHRH-A3)与排卵延迟2组(未注射LHRH-A3)相比,奶牛血清中的P4、E2、LH、FSH和IGF-I的浓度在各个阶段均差异不显著(P0.05);正常组与排卵延迟2组(未注射LHRH-A3)相比,奶牛血清中的P4、E2、LH、FSH和IGF-I的浓度在各个阶段均差异不显著(P0.05),正常组奶牛的血清P4浓度在所有阶段均高于排卵延迟2组(未注射LHRH-A3),但差异不显著(P0.05)。通过对12头临床健康奶牛和20头排卵延迟奶牛进行B超扫查,获得了正常健康和排卵延迟奶牛黄体形成过程中不同阶段的黄体典型声像图,正常组与卵延迟组奶牛相比,黄体相关指标(直径、周长、面积和体积)在配种后7-15天内的变化规律,差异不显著,(P0.05)。应用优化方案治疗效果最好,治愈率为70%,其次为中药理囊,治愈率为50%,常规激素LHRH-A3治疗效果最差,治愈率为37.5%。结论:通过对排卵延迟奶牛从发情到配种后黄体形成过程中的激素(P4、E2、LH、FSH)、细胞因子(IGF-I)、黄体周长、黄体面积、黄体体积以及黄体直径指标变化规律系统的研究,为建立奶牛排卵延迟的诊断方法奠定了基础。应用优化方案治疗奶牛排卵延迟比常规激素LHRH-A3治疗方案、中药理囊临床效果更好,优化治疗方案是排卵延迟奶牛在配种同时注射LHRH-A325μg,在配种后5-7天、15-17天分别肌肉注射长效黄体酮注射液100mg,在配种后28天肌肉注射VADE10ml进行保胎,可以提高受胎率。
[Abstract]:Objective: cows delayed ovulation is one of the large-scale dairy farms in four ovarian disease. This research is based on the actual problems in production practice: Dairy delayed ovulation is not accurate, error diagnosis, treatment is not targeted, and no problem of common miscarriage after mating, thorough the oestrus and ovulation until ovulation during the formation of corpus luteum hormone changes of some cytokines were detected and analyzed, in order to provide the basis for the establishment of diagnosis and miscarriage after delayed ovulation cows breeding. On this basis, the delayed ovulation cows were treated more than the optimal solution. The final purpose is to establish the cow delayed ovulation diagnostic procedures, treatment methods and optimization and improve the pregnancy rate, miscarriage plan, so as to reduce the economic losses of dairy cows. Methods: selected delayed ovulation 12 cows from western animal husbandry dairy clinical center, healthy hair Love ovulation 20 cows, from estrus to 15 days after mating, continuous tracking sampling, ELISA method was used to detect serum collected in P4, E2, LH, FSH and IGF-I concentration, which delayed ovulation group was divided into two groups, respectively, using LHRH-A3 injection (as delayed ovulation group 1) and transmission precision when the injection of LHRH-A3 (as delayed ovulation group 2), analyze the changes of hormone. At the same time in the array B-scanner 7-15 days after mating tracking scan using 50s Tringa Vet portable (2 days every 1 lines), corpus luteum circumference, were measured by electronic scale B-scanner built-in luteal area, luteal volume and the diameter, statistical analysis the variation of corpus luteum index. According to the above study, the diagnostic method of cow delayed ovulation and miscarriage plan, then at the dairy of conventional hormone therapy (LHRH-A3), Chinese medicine treatment group (cyst regulating powder and optimize the treatment plan) chose 60 head of ovulation The cows were delayed clinical observation. Results: the morning of the second day delayed ovulation group 1 (with LHRH-A3) serum LH concentration is lower than the normal group of dairy cows (P0.05), and the difference was significant difference in other stages were not significant (P0.05); the 1 group delayed ovulation (injection of LHRH-A3) and delayed ovulation group 2 (no injection LHRH-A3) compared to cow serum P4, E2, LH, FSH and IGF-I concentrations in each stage were not significant (P0.05); the normal group and delayed ovulation group 2 (without LHRH-A3) than in cow serum P4, E2, LH, FSH and IGF-I concentrations in each stage were not significant (P0.05), serum concentration of P4 group was higher than that of normal cows delayed ovulation 2 groups at all stages (without LHRH-A3), but the difference was not significant (P0.05). Ultrasound scanning in 12 clinical healthy cows and 20 delayed ovulation cows, got healthy and delayed ovulation cows luteal formation process The typical sonographic luteal phase in different stages, the normal group and delayed group compared to cow eggs, relevant indicators of corpus luteum (diameter, perimeter, area and volume) changes in the 7-15 days after mating period, no significant difference (P0.05). Application of optimization scheme is the best treatment, the cure rate was 70%, followed by traditional Chinese medicine science capsule, the cure rate was 50%, conventional hormone therapy effect of LHRH-A3 is the worst, the cure rate was 37.5%. conclusion: the delayed ovulation cows from estrus to mating in the process of formation of corpus luteum hormone (P4, E2, LH, FSH), cell factor (IGF-I), yellow body circumference, corpus luteum area, changes of system the diameter of luteal volume and luteal index, laid the foundation for the establishment of diagnosis method of cows delayed ovulation. Ovulation delay than the conventional hormone LHRH-A3 treatment regimen in the treatment of traditional Chinese medicine application optimization of cows, physical capsule has better clinical efficacy, optimization of treatment is delayed ovulation in milk At the same time in the breeding cows injected with G LHRH-A325, in 5-7 days after mating, 15-17 days respectively, intramuscular injection of long-acting Progesterone Injection 100mg in 28 days after mating, intramuscular injection of VADE10ml can improve the pregnancy rate of miscarriage,.

【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

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