光周期对于绒山羊毛囊干细胞激活的影响

发布时间：2018-03-12 07:20

本文选题：绒山羊　切入点：短日照　出处：《中国畜牧兽医》2017年04期 　论文类型：期刊论文

【摘要】：为了延长绒山羊毛囊兴盛期,提高羊绒产量,本试验通过人工缩短内蒙古阿尔巴斯型绒山羊的日照时间,利用组织切片技术对比人工短光照和自然光照周期下绒山羊皮肤组织形态变化,利用免疫组织化学方法研究毛囊细胞增殖及相关信号通路重要蛋白的表达。结果显示,人工短光照周期下绒山羊毛囊细胞增殖强烈,干细胞标记角蛋白15(cytokeratin 15,K15)阳性信号大量分布于外根鞘,β-catenin信号活跃表达于毛母质及根鞘,毛囊提前进入兴盛期;而自然光照周期下绒山羊毛囊仍停留在休止期,相应蛋白信号表达较弱。综上表明,短日照对毛囊生长具有明显的促进作用,人工短光照周期可以提前激活毛囊干细胞,使其提前进入兴盛期,引起多种毛囊生长相关蛋白的表达。
[Abstract]:In order to prolong the prosperous period of cashmere goat hair follicles and increase cashmere yield, the sunshine time of Inner Mongolia Albanian cashmere goat was artificially shortened. Tissue section technique was used to compare the morphological changes of Cashmere Goat skin under artificial and natural light periods. The proliferation of hair follicle cells and the expression of important proteins related to signal pathway were studied by immunohistochemical method. The proliferation of hair follicle cells of cashmere goats was strong under the artificial short light cycle, the positive signal of stem cell labeled keratin 15 cytokeratin 15 K15) was distributed in the outer root sheath, 尾 -catenin signal was active in the hair matrix and root sheath, and the hair follicle entered the prosperous stage ahead of time. However, the hair follicles of cashmere goats still remained in the resting period under natural light cycle, and the corresponding protein signal expression was weak. The results showed that short sunlight could promote the growth of hair follicles, and artificial short illumination cycle could activate hair follicle stem cells in advance. It can advance to the prosperous stage and induce the expression of many kinds of hair follicle growth-related proteins.
【作者单位】：内蒙古自治区农牧业科学院;中国科学院昆明动物研究所;内蒙古农业大学;鄂托克前旗北极神绒牧业研究所;
【基金】：国家自然科学基金项目(31260545) 国家公益性行业(农业)科研专项(201303059) 内蒙古自治区自然科学基金项目(2014BS0333) 内蒙古自治区创新基金项目(2013CXJJM03) 内蒙古农牧业科学院青年创新基金项目(2013QNJJM05) 内蒙古自然科学基金重大项目(2013ZD06)
【分类号】：S827

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