免疫增强剂提高禽用二联苗免疫效力的研究

发布时间：2018-03-12 14:01

本文选题：免疫增强剂　切入点：新城疫-传染性法氏囊二联灭活苗　出处：《中国农业科技导报》2016年01期 　论文类型：期刊论文

【摘要】：为评价免疫增强剂(VA5)对禽用联苗的免疫增强作用,将VA5分别与市售鸡新城疫-传染性法氏囊(新法)二联苗合用免疫SPF鸡和蛋鸡、与新城疫-H9亚型禽流感(新流)二联苗合用仅免疫SPF鸡,评价免疫后的抗体效价和SPF鸡攻毒后的保护效力。结果显示,SPF鸡或蛋鸡免疫含VA5的新法二联苗、新流二联苗后,新城疫血凝抑制(HI)抗体效价比常规苗组提前1周达到6 log2,禽流感HI抗体效价比常规苗组提前1周达到7 log2,法氏囊血清琼扩抗体和中和抗体比常规苗组均提前1周合格,且3种抗体持续高于常规疫苗组。免疫含VA5疫苗组的SPF鸡对新城疫强毒攻毒,以临床症状判断,可达全保护;以囊病变和临床症状判断,对法氏囊的攻毒提供全保护;H9变异株攻毒后不排毒。对上述3种病毒的保护力均高于常规苗。上述结果表明,VA5免疫增强剂能够提高鸡新法二联苗、新流二联苗对鸡的免疫效力,具有很好的应用前景。
[Abstract]:In order to evaluate the immune enhancement effect of VA5) on avian vaccine, SPF and laying hens were immunized with VA5 combined with Newcastle disease (NDV) and infectious bursal bursa (IBD) vaccine respectively. Only SPF chickens were immunized with Newcastle disease H9 subtype avian influenza vaccine. The antibody titers after immunization and the protective effect of SPF chickens were evaluated. The results showed that SPF chickens or layers were immunized with the new method double vaccine containing VA5, and after the new flow double vaccine, the antibody titers and the protective effect of SPF chickens were evaluated. The titer of HI antibody of Newcastle disease was 6 log2 weeks earlier than that of routine vaccine group, HI antibody titer of avian influenza reached 7 log2 weeks earlier than that of routine vaccine group, and the titer of antibody and neutralizing antibody in serum of bursa of Fabricius was 1 week earlier than that of routine vaccine group. The SPF chickens immunized with VA5 vaccine could be completely protected against Newcastle disease (NDV) by clinical symptom judgement, and by cystic disease and clinical symptoms. The total protection against bursa of bursa was provided to H9 mutant strain without detoxification. The protective power of the above three viruses was higher than that of conventional vaccine. The results showed that VA5 immunopotentiator could improve the immune efficacy of the new vaccine and the new lipids vaccine against chickens. It has a good application prospect.
【作者单位】：江苏省农业科学院国家兽用生物制品工程技术研究中心农业部兽用生物制品工程技术重点实验室;南京天邦生物科技有限公司;江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：国家自然基金项目(31201904) 国家863计划项目(2011AA10A213) 江苏省农业科技自主创新项目[CX(14)5045] 中央财政农业技术推广资金[TG(14)067]资助
【分类号】：S852.4

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