大肠杆菌耐药性及氟苯尼考耐药基因floR的研究

发布时间：2018-03-12 16:13

本文选题：大肠杆菌　切入点：耐药性　出处：《中国畜牧杂志》2017年01期 　论文类型：期刊论文

【摘要】：本实验对某地养殖场猪源和鸡源大肠杆菌的耐药性及氟苯尼考耐药基因flo R进行分析,旨在了解我国大肠杆菌的耐药现状,以降低对养殖业的危害,减少菌株耐药性的传播,为兽医临床用药及兽药管理提供科学依据,为新型抗菌药物的研制奠定理论基础。采用国标法分离鉴定大肠杆菌,肉汤微量稀释法进行药敏实验,PCR法检测氟苯尼考flo R基因,膜过滤法进行转化接合实验。结果表明:共分离大肠杆菌293株,分离率为73.3%,其中猪源157株,鸡源136株;大肠杆菌对磺胺类、四环素类和氨基糖苷类药物的耐药性相对严重,对氯霉素类、氟喹诺酮类和β-内酰胺类药物相对敏感;与欧盟EUCAST标准相比,该地区大肠杆菌的MIC分布出现明显右移现象;80%以上的大肠杆菌均为多重耐药菌株,以8重和9重耐药菌株为主;氟苯尼考耐药基因flo R携带率为61.2%,flo R基因可以在菌株间相互转移。样品采集地区养殖场大肠杆菌污染严重,耐药种类逐渐增多,多重耐药现象严重,质粒介导的耐药基因可以在菌株间相互转移。
[Abstract]:In this experiment, the resistance of Escherichia coli and florfenicol resistance gene flo R from pig and chicken were analyzed in order to understand the current situation of drug resistance of Escherichia coli in China, in order to reduce the harm to the breeding industry and reduce the spread of drug resistance of strains. To provide scientific basis for veterinary clinical drug use and veterinary drug management, and to lay a theoretical foundation for the development of new antimicrobial agents, Escherichia coli was isolated and identified by national standard method, and florfenicol flo R gene was detected by broth microdilution assay. The results showed that 293 strains of Escherichia coli were isolated, and the isolation rate was 73.3%, of which 157 strains were from pigs and 136 strains from chicken, and the resistance of Escherichia coli to sulfonamides, tetracyclines and aminoglycosides was relatively serious. Compared with the European Union EUCAST standard, the MIC distribution of Escherichia coli in this area showed obvious right shift phenomenon, more than 80% of the Escherichia coli were multidrug resistant strains, which were sensitive to chloramphenicol, fluoroquinolones and 尾 -lactams. The flo R carrying rate of florfenicol resistance gene was 61.2% and that of floro R gene could be transferred from strain to strain. The contamination of Escherichia coli in culture farm was serious in the area of sample collection, and the kinds of drug resistance increased gradually. Multidrug resistance is serious and plasmid-mediated resistance genes can be transferred from strain to strain.
【作者单位】：沈阳农业大学生物科学技术学院;中国动物疫病预防控制中心动物安全检测室;沈阳农业大学工程学院;
【分类号】：S852.612

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