一株马流感病毒的基因序列测定分析及Donegal毒株的生物学特性研究

发布时间：2018-03-12 18:31

本文选题：马流感病毒　切入点：同源性　出处：《内蒙古农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：马流行性感冒,是由正粘病毒科流感病毒属马流感病毒所引起的,主要导致马属动物的急性暴发式传染病。近几年来,马流感呈全球流行状态,仅有部分岛国未发病;我国也未能避免,历史上共发生过四次马流感疫情。随着我国马产业逐渐的发展壮大,一旦再次爆发大范围的马流感疫情,将会给我国马产业造成重创。本研究通过对一匹患病马进行病料采集及后续的病毒分离、病毒核酸的提取、RT-PCR、连接T载体等一系列分子生物学技术,最终对病毒核酸进行测序。经测序比对分析,本次研究获得一株H3N8亚型马流感病毒。将获得的毒株与我国分离的A/equine/Jilin/1/1989(H3N3)禽源性毒株、2002年美国肯塔基分离株A/equine/Kentucky/1/02(H3N8)(美洲谱系Florida亚型)、A/equine/Argentine/77(H3N8)(美洲谱系Argentine亚型)这几株有代表性的毒株进行同源性分析后,发现该毒株与Florida-2型的马流感病毒基因片段的同源性最高。根据马流感病毒的命名规则,我们将这株毒命名为A/equine/Heilongjiang/1/2010(H3N8)。本次所分离到的黑龙江株马流感病毒,丰富了我国马流感病毒资源库,为我国马流感流行病学研究、诊断技术及疫苗研究提供了重要基础。为了预防国外流行的Florida-1型马流感病毒传入我国,同时对Florida-1型马流感病毒有基本的了解,本研究复苏了Florida-1型的毒株A/equine/Donegal/1/2009(H3N8),该毒株于2009年在爱尔兰分离得到。经过一系列的试验之后,发现该毒株具有较强的耐受性,其血凝素的热稳定性较好。
[Abstract]:Equine influenza is caused by the influenza virus of the family Orthomyxoviridae, which mainly causes an acute outbreak of infectious diseases in equine animals. In recent years, equine influenza is in a global epidemic state, and only some island countries have not suffered from it. China is also unable to avoid, there have been four horse flu outbreaks in history. With the gradual development of the horse industry in our country, once a large-scale equine influenza epidemic occurs again, In this study, a series of molecular biological techniques, such as collection of diseased horse and subsequent virus isolation, extraction of virus nucleic acid RT-PCR, ligation of T vector, and so on, will cause severe damage to the horse industry in China. Finally, the viral nucleic acid was sequenced. In this study, a strain of H3N8 subtype of equine influenza virus was obtained, and the strain of avian origin, A / P / Jilin / R / 1 / 1989 / H3N3, isolated from China, and in 2002, A / P / Kent / 1 / 02 / H3N8 (American strain of Florida subtype) was a typical strain of Aequine / Argentine / 77H3N8 (American lineage Argentine subtype). After homology analysis, It was found that the strain had the highest homology with the equine influenza virus Florida-2 gene fragment. According to the naming rules of the equine influenza virus, we named the virus Ar / equine / Heilongjiang / Heilongjiang / 2010 / H3N8 virus. The Heilongjiang strain of equine influenza virus isolated this time, It has enriched the resource bank of equine influenza virus in China, and provided an important foundation for the epidemiological study, diagnostic technology and vaccine research of equine influenza in China. In order to prevent the introduction of Florida-1 influenza virus from abroad into China, At the same time, we have a basic understanding of the Florida-1 equine influenza virus. In this study, we resuscitated the strain of Florida-1 type A / Q / T / 2009 H3N8, which was isolated in Ireland in 2009. After a series of tests, we found that the strain has strong tolerance. Its hemagglutinin has good thermal stability.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.65

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