AFB1诱导肉鸡亚慢性肝损伤模型的建立及姜黄素的干预效果

发布时间：2018-03-23 06:07

本文选题：AFB1　切入点：亚慢性肝损伤　出处：《东北农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：肝脏是机体内重要的解毒器官,摄入的毒物吸收后进入肝脏通过生物转化起到解毒或活化的作用。所以肝脏易受内外源性毒物的侵害造成肝损伤。黄曲霉毒素B1(AFB1)是黄曲霉毒素(AF)中的一种,具有极强的肝毒性和致癌性。肉鸡对AFB1易感。深入研究AFB1致肉鸡肝损伤的发病机理,积极开发经济价值高、活性强、疗效好的保肝药物是我们重要的科研目标。姜黄素是姜黄的主要活性成分,是天然的食品添加剂、抗氧化剂和抗炎药物。本试验选择国内广泛饲养的AA肉鸡作为试验动物,以临床症状、肝体指数、血清转氨酶(ALT、AST)、肝组织氧化应激指标(GSH-Px、SOD、MDA)及病理组织学作为评价指标,在筛选AFB1诱导AA肉鸡亚慢性肝损伤的最佳浓度和时间的基础上,建立AFB1诱导的AA肉鸡亚慢性肝损伤模型。通过染毒的同时干预不同剂量的姜黄素,分析姜黄素对AFB1诱导的AA肉鸡亚慢性肝损伤的干预效果。本试验结果对防治AA肉鸡AFB1亚慢性中毒,研究AFB1致毒机制以及姜黄素在兽医临床上的应用提供理论依据。研究结果表明:1、AFB1诱导AA肉鸡亚慢性肝损伤模型的建立采用高(5 mg·kg~(-1))、中(3 mg·kg~(-1))、低(1 mg·kg~(-1))剂量AFB1添加入基础饲料中,连续染毒AFB1 14 d、21 d、28 d。结果显示,连续染毒28d,AFB1高剂量组鸡肝体指数显著高于空白对照组(P0.05);血清转氨酶活性极显著高于空白对照组(P0.01);肝组织内抗氧化酶活性极显著低于空白对照组(P0.01),氧化产物含量极显著高于空白对照组(P0.01)。病理组织学结果显示,AFB1高剂量组肝脏结构紊乱,肝脏局部纤维化,血管内淤血,肝索结构模糊,肝细胞空泡变性,淋巴细胞浸润。中、高剂量AFB1能够显著降低AA肉鸡平均日增重。提示连续28 d饲喂含有5 mg·kg~(-1) AFB1的饲料可以成功建立AA肉鸡亚慢性肝损伤模型,AA肉鸡出现明显AFB1中毒症状、体重降低。2、姜黄素对AFB1致AA肉鸡亚慢性肝损伤的干预效果本试验以临床症状、肝体指数、血清转氨酶活性、肝脏氧化应激指标及病理组织学结果为检测项目,对饲料中添加不同剂量姜黄素(150 mg·kg~(-1)、300 mg·kg~(-1)和450 mg·kg~(-1))对AFB1诱导的AA肉鸡亚慢性肝损伤的干预效果进行评价。连续染毒及干预28 d,与模型组相比,姜黄素干预后,AA肉鸡AFB1中毒症状减少,平均日增重增加,肝损伤明显减轻,肝损伤缓解程度与姜黄素剂量呈正相关。说明姜黄素连续干预的情况下,姜黄素对AFB1诱导的AA肉鸡亚慢性肝损伤具有预防性的保护作用。停止染毒和姜黄素干预后,试验35 d,AFB1模型组肝脏仍存在明显的肝损伤,高剂量姜黄素仍对AFB1诱导的AA肉鸡亚慢性肝损伤存在持续的保护作用。以上结果表明,以5 mg·kg~(-1)剂量将AFB1拌入料中,连续饲喂染毒饲料28 d可成功建立AFB1诱导AA肉鸡亚慢性肝损伤模型;姜黄素能够有效拮抗AFB1所致AA肉鸡亚慢性肝损伤,起到保护肝脏的作用,尤其是高剂量姜黄素的保护作用较为持久,可以有效缓解AFB1的毒性。建议混饲的姜黄素干预剂量为450 mg·kg~(-1)。
[Abstract]:The liver is an important detoxification organ in the body, intake of poison absorbed into the liver through biotransformation to detoxification or activation. So the liver is susceptible to exogenous toxins damage caused liver injury. Aflatoxin B1 (AFB1) is yellow aspergillus toxin (AF) in one, with strong liver toxicity and carcinogenicity. Chickens are susceptible to AFB1. The pathogenesis of liver injury caused by meat AFB1 is studied deeply, and actively develop high economic value, high activity, good effect of hepatoprotective drugs is our important research goal. Curcumin is the main active component of turmeric, is a natural food additive, antioxidant and anti-inflammatory drugs. This experiment a wide choice of domestic AA broiler breeding as test animal, with clinical symptoms, liver index, serum transaminase (ALT, AST), the index of oxidative stress in liver tissue (GSH-Px, SOD, MDA) and histopathology as the evaluation index, the selection of AFB 1 AA broilers induced by sub chronic liver injury of optimal concentration and time, the establishment of AA broiler sub chronic liver injury model induced by AFB1. Different doses of curcumin through exposure and intervention, analysis of curcumin intervention effect on the injury of AA broiler sub chronic liver induced by AFB1. The test results on the prevention and treatment of AA broilers AFB1 chronic poisoning and provide a theoretical basis for the application of AFB1 and the toxic mechanism of curcumin in veterinary clinic. The results showed that: 1, the establishment of the AA chicken sub chronic liver injury model was induced by high AFB1 (5 mg - kg~ (-1), (3) in mg kg~ (-1), low (1 mg) kg~ (-1)) dose of AFB1 added to the basal diet, and continuous exposure to AFB1 14 d, 21 d, 28 d. results showed that continuous exposure to 28d, AFB1 high dose group liver index were significantly higher than the control group (P0.05); serum transaminase activity was significantly higher than control group (P0.01); liver tissue Anti oxidase activity was significantly lower than control group (P0.01), the oxidation product content was significantly higher than the control group (P0.01). The pathological results showed that high dose of AFB1 group of liver structure disorder, liver fibrosis, vascular congestion, livercable structure fuzzy, liver cell degeneration, lymphocyte infiltration, high doses of AFB1 AA can significantly reduce the average daily gain of broiler. The results suggest that the 28 d feed containing 5 mg (-1) - kg~ AFB1 feed can be successfully established AA broiler sub chronic liver injury model, AA broilers appeared AFB1 poisoning symptoms, body weight decreased.2, intervention effect of curcumin on AFB1 induced sub chronic liver injury of AA broilers in the test the clinical symptoms, liver index, serum transaminase activity and oxidative stress in the liver index and histopathological results for the detection of the project, adding different doses of dietary curcumin (150 mg - kg~ (-1), 300 mg - kg~ (-1) and 4 50 mg - kg~ (-1)) to evaluate the intervention effect on the injury of AA broiler sub chronic liver induced by AFB1. Continuous exposure and 28 d intervention, compared with the model group, curcumin intervention, AA broiler AFB1 poisoning symptoms decreased, average daily gain, liver damage significantly reduced liver injury relief degree and curcumin the dose was positively correlated. Curcumin intervention, curcumin has a protective effect on the injury of AA broiler preventive sub AFB1 induced chronic liver. Stop exposure and curcumin intervention, 35 d test, AFB1 model group liver still exist significant liver injury, high dose of curcumin has protective effect on the injury of AA continuous broiler sub chronic liver induced by AFB1. The above results showed that 5 mg - kg~ (-1) AFB1 dose mix material, continuously fed contaminated feed 28 d can be successfully established by AFB1 AA broilers sub chronic liver injury model; curcumin can effectively Antagonizing the subchronic liver injury induced by AFB1 in AA broilers, it plays a protective role in liver. Especially, the protective effect of high dose curcumin is more durable, which can effectively alleviate the toxicity of AFB1. It is suggested that the dosage of curcumin in mixed feeding is 450 mg kg~ (-1).

【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S859.8

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