不同乳酸菌培养物对肉鸡生产性能及免疫功能的影响

发布时间：2018-03-28 18:56

本文选题：乳酸菌培养物　切入点：肉鸡　出处：《吉林农业大学》2015年硕士论文

【摘要】：本试验采用正交试验方法,通过测定三种乳酸菌发酵液的抑菌活性,筛选出三种对致病菌有抑制作用的乳酸菌培养物,研究其对肉鸡生产性能、消化机能、盲肠微生物、免疫功能、抗氧化性能及血清生化指标的影响。采用单因子完全随机化试验设计,将体重相近的健康1日龄AA肉仔鸡300只随机分配到5个处理组中。其中对照组(C)饲喂基础日粮,抗生素组(A)在基础日粮中添加0.01%金霉素,试验组L1、L2、L3分别在基础日粮中添加0.16%乳酸菌培养物L1、L2、L3。每个处理6个重复,每个重复10只鸡。试验期为42天,分别于21日龄和42日龄屠宰取样。结果表明乳酸菌培养物L1显著提高肉鸡平均日采食量(P0.01)和料重比(P0.05),L3显著提高试验前期平均日增重(P0.01),L1和L2提高试验后期平均日增重(P0.01)。日粮中添加乳酸菌培养物L1能显著提高试验前期肉鸡免疫器官指数、盲肠乙酸浓度和血清中SOD活性,显著提高试验后期肝脏、胰腺指数、磷消化率(P0.05)和血清总蛋白、白蛋白数量及ALP活性(P0.01)。日粮中添加乳酸菌培养物L2能显著提高试验前期肉鸡血清总蛋白、白蛋白数量(P0.01),显著提高整个试验期肝脏指数及试验后期肉鸡免疫器官指数、盲肠丙酸和总VFA浓度、血清新城疫抗体效价、T-AOC和ALP活性(P0.05)。日粮中添加乳酸菌培养物L3能显著提高试验前期肉鸡粗脂肪消化率、脾脏指数和血清中SOD活性,显著提高试验后期胸腺指数、血清中IgA含量(P0.05)和整个试验期血清中ALP活性(P0.01)。日粮中添加乳酸菌培养物能显著降低盲肠乳酸杆菌数量(P0.05)和大肠杆菌数量(P0.01),显著提高盲肠双歧杆菌数量和试验后期氮的表观沉积率(P0.05)。综上所述,在日粮中添加灭活的乳酸菌培养物,L3的抑菌效果最好,试验前期添加量适宜,试验后期添加量应适当降低;L1和L2的抑菌效果较差,试验前期添加量应适当增加,试验后期添加量适宜。在肉鸡生产中,可用0.16%灭活乳酸菌培养物替代抗生素,从而达到更好的经济效果。
[Abstract]:By means of orthogonal test, three kinds of lactic acid bacteria culture were screened out by determining the bacteriostatic activity of three kinds of lactic acid bacteria fermentation broth, and the performance, digestion function and cecum microorganism of three kinds of lactic acid bacteria culture were studied. Effects of immune function, Antioxidant activity and Serum biochemical Indexes. Three hundred healthy 1-day-old AA broilers of similar body weight were randomly assigned to five treatment groups. The control group (C) was fed with basal diet, the antibiotic group (A) added 0.01% chlortetracycline into the basal diet. In the trial group, L1 + L2N L3 was added to the basal diet with 0.16% lactic acid bacteria culture L1 / L2 + L3. Each treatment included 6 replicates and 10 chickens per replica. The trial period was 42 days. The results showed that Lactic acid bacteria culture L1 significantly increased the average daily feed intake (P 0.01) and feed weight ratio (P 0.05) L 3 significantly increased the average daily gain (P 0.01 L 1 and L 2) in the early stage of the experiment. Lactic acid bacteria culture L1 could significantly increase the immune organ index of broilers in the early stage of the experiment. The concentration of acetic acid in cecum and the activity of SOD in serum significantly increased liver, pancreas index, phosphorus digestibility (P 0.05) and serum total protein. The amount of albumin and the activity of ALP were significantly increased by adding lactic acid bacteria culture L2 to the diet. The serum total protein and albumin content of broilers were significantly increased, and the liver index and immune organ index of broilers were significantly increased during the whole trial period. The concentration of propionic acid and total VFA in the caecum, the titer of serum Newcastle disease antibody, the activity of T-AOC and the activity of ALP were P0.050.The dietary supplementation of Lactic acid bacteria culture L3 could significantly increase the digestibility of crude fat, spleen index and the activity of SOD in serum of broilers in the early stage of the experiment. The thymus index was significantly increased in the later stage of the trial. The content of IgA in serum and the activity of ALP in serum during the whole trial period were P0.010.The addition of lactic acid bacteria to the diet significantly decreased the number of Lactobacillus caecum and Escherichia coli, and significantly increased the number of bifidobacterium cecum and the later stage of the experiment. The apparent deposition rate of nitrogen is P0.050.In summary, The bacteriostatic effect of inactivated lactic acid bacteria culture (L3) was the best, the amount of L3 was suitable in the early stage of the experiment, the addition amount of L2 and L2 should be reduced in the later stage of the experiment, and the amount added in the early stage of the experiment should be increased appropriately. In the later stage of the experiment, the antibiotics could be replaced by 0.16% inactivated lactic acid bacteria culture in the broiler production, thus achieving a better economic effect.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5

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