猪源荚膜血清F型多杀性巴氏杆菌的分离鉴定、毒力基因检测与致病性研究

发布时间：2018-03-31 22:02

本文选题：多杀性巴氏杆菌　切入点：荚膜血清F型　出处：《中国预防兽医学报》2017年03期

【摘要】：为调查多杀性巴氏杆菌(P.multocida)在湖南猪群中的流行情况,本实验室于2015年6月~2016年3月在湖南大型屠宰场采集猪肺脏222份和猪扁桃体180份临床样品,采用血琼脂培养和PCR方法进行P.multocida的分离鉴定,得到4株F型P.multocida。对分离的4株F型P.multocida进行毒力基因检测以及小鼠的致病性研究,结果显示4株菌均含有ptf A、pfhA、fim A、sodA、sodC、ompA、ompH、oma87、plpB、exbB、exbD、tonB、hgbB、Fur、nan H、hsf-1和hsf-2毒力基因,但tadD、toxA、hgbA、nanB、pm HAS、tbpA毒力基因均为阴性;4株菌致病性试验结果显示,感染小鼠的剂量分别为4 cfu/只、5 cfu/只、11 cfu/只和17 cfu/只时可导致小鼠在60 h内全部死亡,表明这4株菌对小鼠有强致病力。病理切片显示,感染小鼠有明显的肺泡充血、脾淋巴细胞凋亡、肾小管和肾小球萎缩等病理变化。本实验首次在湖南地区分离到猪源荚膜血清F型P.multocida,并进行了较为详细的生物学特性研究,为国内P.multocida的流行情况和生猪多杀性巴氏杆菌病的防控补充了参考资料。
[Abstract]:In order to investigate the prevalence of P. multocida in pigs in Hunan Province, 222 pig lungs and 180 clinical samples of porcine tonsils were collected from Hunan slaughterhouse from June 2015 to March 2016. By using blood Agar culture and PCR method, four strains of F type P.multocida.The virulence genes of four F type P.multocida and pathogenicity of mice were studied. The results showed that all the four strains contained ptf pfhAfim sodAsodAsodCompAompAoma87plpBnexbBmexbBnexbBnexhbBnexhsf-1 and hsf-2 virulence genes. However, the pathogenicity test of TDD toxin hgbANANBnBnbPm HASTBPA virulence genes showed that the dose of 4 cfu/, 5 cfu/, 11 cfu/ and 17 cfu/ could lead to the death of all the mice within 60 h. The pathological sections showed that the infected mice had obvious alveolar congestion and splenic lymphocyte apoptosis. The porcine perfringent serum type F, P.multocida, was isolated for the first time in Hunan province, and the biological characteristics of the serum were studied in detail, including the pathological changes of renal tubules and glomerular atrophy. It provides references for the prevalence of P.multocida in China and the prevention and control of porcine pasteurellosis.
【作者单位】：湖南农业大学动物医学院;
【基金】：家畜疫病病原生物学国家重点实验室开放基金课题(SKLVEB2013KFKT002) 湖南省战略性新兴产业科技攻关项目(2016GK4015)
【分类号】：S852.61

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