单色绿光对鸡胚褪黑激素合成的影响

发布时间：2018-04-03 12:48

  本文选题：单色绿光　切入点：褪黑激素　出处：《畜牧兽医学报》2017年05期

【摘要】：本研究旨在探讨单色绿光对鸡胚松果体发育以及褪黑激素合成的影响,为研究单色绿光影响鸡胚发育提供依据,选用120枚质量为(65±3)g的受精鸡胚,随机分为两组,分别在绿光(波长560nm,光强15lx)和黑暗条件下孵化,每组设4个重复,每个重复5枚鸡胚,分别在E(胚龄)15、E18和E21取材,探索鸡胚孵化过程中单色绿光对松果体的发育以及褪黑激素合成的影响。结果表明:(1)孵化期间给予绿光照射,鸡胚质量增加11.19%~21.41%(P0.05);(2)单色绿光促进松果体小叶发育;(3)褪黑激素合成关键酶——乙酰基转移酶(AANAT)转录水平的表达随着胚龄的增加而升高,其中黑暗组E21时AANAT mRNA表达较E15和E18时显著高29.84%~47.06%(P0.05),但是E15与E18间差异不显著(P0.05);而绿光组中,E18 mRNA水平较E15 AANAT mRNA的表达显著高17.76%(P0.05),E21时较E15和E18时松果体中AANAT mRNA表达水平显著高26.37%~48.81%(P0.05);并且单色绿光组高于黑暗组12.85%~15.95%(E18~E21,P0.05);(4)血浆褪黑激素含量随着胚龄的增加而升高,黑暗组E21褪黑激素含量为E15和E18的2.6和1.9倍,绿光组中E21为E15和E18的2.8和1.8倍;在E21和E18,绿光组鸡胚外周血褪黑激素水平高于黑暗组的13.13%和18.20%(P0.05),并且与外周血中生长激素水平极显著正相关(P0.001)。由此可知,在鸡胚孵化期间给予单色绿光刺激可促进松果体发育以及褪黑激素的合成与释放,进而促进生长激素水平,最终促进鸡胚发育。
[Abstract]:The purpose of this study was to investigate the effects of monochromatic green light on the pineal development and melatonin synthesis of chicken embryos. In order to study the effect of monochromatic green light on the development of chicken embryos, 120 fertilized chicken embryos with a mass of 65 卤3 g were randomly divided into two groups.Hatched under the conditions of green light (wavelength 560 nm, light intensity 15lx) and dark conditions, each group was divided into 4 replicates, each replicating 5 chicken embryos.To explore the effect of monochromatic green light on pineal development and melatonin synthesis during hatching of chicken embryo.The results showed that the weight of chicken embryo increased by 11.19% and 21.41% during hatching (P0.05) green light promoted the development of pineal lobule. (3) the expression of key enzyme of melatonin biosynthesis, acetyltransferase (AANAT2), increased with the increase of embryo age.鍏朵腑榛戞殫缁凟21鏃禔ANAT mRNA琛ㄨ揪杈僂15鍜孍18鏃舵樉钁楅珮29.84%~47.06%(P0.05),浣嗘槸E15涓嶦18闂村樊寮備笉鏄捐憲(P0.05);鑰岀豢鍏夌粍涓，

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