不同年龄山羊胃肠道乳酸菌的分离鉴定

发布时间：2018-04-27 02:39

  本文选题：山羊 + 乳酸菌　； 参考：《黑龙江畜牧兽医》2017年05期

【摘要】：为了解不同年龄山羊胃肠道乳酸菌种类的差异,试验对2月龄断奶羔羊、8月龄和2岁山羊瘤胃刮取物及回肠黏膜刮取物中的乳酸菌进行了分离,并鉴定了不同年龄山羊乳酸菌的种类。结果表明:从2月龄断奶羔羊瘤胃刮取物中分离到巴黎链球菌,回肠黏膜刮取物中分离到屎肠球菌;8月龄山羊瘤胃刮取物中分离到巴黎链球菌、耐久肠球菌和弯曲乳杆菌,回肠黏膜刮取物中分离到屎肠球菌和肠膜明串珠菌;从2岁山羊瘤胃刮取物中分离到巴黎链球菌、耐久肠球菌、戊糖片球菌、海氏肠球菌、鹑鸡肠球菌,回肠黏膜刮取物中分离到屎肠球菌、肠膜明串珠菌和粪肠球菌。说明随着山羊年龄的增加,瘤胃黏膜和回肠黏膜中乳酸菌的种类增加,可从较大年龄山羊分离鉴定的乳酸菌中筛选出作为益生菌的菌株用以研究和开发山羊微生态制剂供羔羊使用。
[Abstract]:In order to understand the different types of lactic acid bacteria in gastrointestinal tract of goats of different ages, the two month old weaned lambs were isolated from rumen scraping and ileal mucosal scraping. The species of lactic acid bacteria in goats of different ages were identified. The results showed that Streptococcus Paris was isolated from rumen scraping of 2-month-old weaned lambs and Streptococcus faecium from 8-month-old goat rumen scraping was isolated from ileal mucosal scraping, and Streptococcus mutans, Enterococcus durum and Lactobacillus campylobacter were isolated from the rumen scrapes of weaned lambs at 2 months old. Enterococcus faecium and Streptococcus enteromembranus were isolated from ileal mucosal scraping, and Streptococcus Paris, Enterococcus duris, Staphylococcus pentose, Enterococcus hepari and Enterococcus quail were isolated from rumen scraping of 2-year-old goat. Enterococcus faecium, Streptococcus faecalis and Enterococcus faecalis were isolated from ileal mucosal scraping. The results showed that the species of lactic acid bacteria in rumen mucosa and ileum mucosa increased with the increase of goat age. Strains of lactic acid bacteria isolated and identified from older goats as probiotics can be used to study and develop goat microecological preparations for use by lambs.
【作者单位】： 西南民族大学生命科学与技术学院;
【基金】：国家民委科技项目(14XNZ025) 中央高校基本科研业务费专项(2014NZYQN58) 四川省教改项目“兽医专业学位研究生教育实践基地建设”
【分类号】：S827
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本文编号：1808838