一株犬种布鲁氏菌的鉴定及毒力测定

发布时间：2018-04-27 17:52

  本文选题：犬种布鲁氏菌 + 分离鉴定　； 参考：《中国兽药杂志》2017年09期

【摘要】：对分离到的一株疑似犬种布鲁氏菌进行生物特性和基因型鉴定,并用宿主实验动物(比格犬)测定了最小感染量和脾含菌量。形态与染色特性、培养特性、血清学特性、生化特性鉴定以及Multi-PCR和16S rRNA测序结果表明,该分离株为犬种布鲁氏菌,其对比格犬的最小感染剂量为10~5 CFU/m L,对应的脾含菌量为4×10~5~3×10~6 CFU/g。
[Abstract]:The biological characteristics and genotypes of a suspected canine brucella strain were identified and the minimal infection and spleen bacterial content were determined by the host laboratory animal (Beagle dog). The results of morphological and staining characteristics, culture characteristics, serological characteristics, biochemical characteristics and Multi-PCR and 16s rRNA sequencing showed that the strain was Brucella canis, and the minimum infection dose to beagle was 105 CFU/m / L, and the corresponding spleen contained 4 脳 10 ~ (5) ~ (5) C ~ (-1) C ~ (-1) C ~ (-1) ~ (-1) ~ (-1) ~ 3 脳 10 ~ (-6) CFU ~ (-1) 路g.
【作者单位】： 北京农学院;中国兽医药品监察所;北京市畜牧兽医总站;
【分类号】：S852.61
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本文编号：1811710