3种高致病性猪蓝耳病疫苗对仔猪免疫器官的损伤作用

发布时间：2018-05-01 11:26

本文选题：猪蓝耳病 + 疫苗　；参考：《华南农业大学学报》2017年04期

【摘要】：【目的】研究3种高致病性猪蓝耳病(PRRS)疫苗(MLV、TJM92、JXA-1R)对仔猪免疫器官的损伤作用。【方法】选取50头初生健康仔猪,分为4组进行免疫:未免疫空白对照组(CK组)、MLV疫苗免疫组(A组)、TJM92疫苗免疫组(B组)、JXA-1R疫苗免疫组(C组)。病理剖检后,制作石蜡切片,通过优化的酯酶染色(α-ANE染色)、甲基绿-派洛宁染色(MG-P)方法统计各免疫器官的酯酶阳性T淋巴细胞和浆细胞(效应B淋巴细胞)比率变化。【结果】各免疫组的免疫器官酯酶阳性T淋巴细胞和浆细胞所占百分比与对照组存在显著或极显著差异。【结论】3种疫苗对仔猪免疫器官均有不同程度的损伤作用,具体表现为JXA-1R疫苗损伤作用最强,TJM92疫苗次之,MLV疫苗最弱。
[Abstract]:[objective] to study the effects of three highly pathogenic porcine blue ear disease (PRRS) vaccines, MLV TJM92, JXA-1R, on immune organs of piglets. [methods] 50 newborn healthy piglets were selected. The mice were divided into 4 groups: the control group without immunization (CK group) was immunized with MLV vaccine (group A) and TJM92 vaccine group (group B) was immunized with JXA-1R vaccine (group C). After pathological examination, paraffin sections were made. The ratio of esterase positive T lymphocytes and plasmacytes (effector B lymphocytes) in each immune organ was estimated by the optimized esterase staining (伪 -ANE staining and methyl green-pyronine staining). [results] Immunoimmunity of each immune group The percentages of esterase positive T lymphocytes and plasma cells in epidemic organs were significantly different from those in the control group. [conclusion] the immune organs of piglets were damaged in different degrees by the three vaccines. The damage of JXA-1R vaccine was the strongest and the second was TJM92 vaccine.
【作者单位】：华南农业大学兽医学院;佛山科学技术学院;
【基金】：广东高校畜禽疾病诊断防控技术开发中心资助项目(GCZX-B1102)
【分类号】：S858.28

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