一株猪流感病毒的遗传进化分析及其致病力研究

发布时间：2018-05-08 19:45

本文选题：猪流感 + 遗传进化分析　；参考：《中国预防兽医学报》2017年12期

【摘要】：为研究猪流感病毒(Swine influenza virus,SIV)A/swine/Dezhou/37/2015(H1N1)株的遗传进化和致病力,本研究对该分离株的8个基因节段进行了遗传进化分析,并接种小鼠观察临床表现及检测其肺脏的病理变化情况。结果显示:分离株HA基因的裂解位点为PSIQSR↓G,无多个连续碱性氨基酸插入,为低致病性SIV;HA、M、NA节段处于欧洲类禽H1N1分支;NP、PA、PB1、PB2节段处于2009甲型H1N1分支;NS节段处于北美三源重排分支;对小鼠致病力研究显示该毒株可感染小鼠并引起其肺脏组织病理学变化,但对小鼠无致死性。以上结果表明该分离株为一株低致病性的新型三源重排SIV。本研究为山东地区SIV的流行特点及变异情况提供了实验依据。
[Abstract]:In order to study the genetic evolution and pathogenicity of the Swine influenza virus (SIV) A/swine/Dezhou/37/2015 (H1N1) strain, the genetic evolution of 8 segments of the isolate was analyzed in this study. The clinical manifestations and pathological changes of the lungs were observed in mice. The results showed that the cleavage site of the HA gene of the isolated strain was found. For PSIQSR G, no successive alkaline amino acids were inserted into the low pathogenic SIV; HA, M, NA segments were in the European avian H1N1 branch; NP, PA, PB1, PB2 segments were in the 2009 type H1N1 branch; the segment was in the three source rearrangement branch of North America; the study of virulence in mice showed that the virus could infect mice and cause pathological changes of lung tissue, but against the pathological changes of lung tissue, but the The results showed that the isolated strain was a new type of low pathogenic three source rearrangement SIV., which provided experimental basis for the epidemic characteristics and variation of SIV in Shandong area.

【作者单位】：山东农业大学动物科技学院;山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室;得利斯集团有限公司;
【基金】：山东省博士基金(BS2013SW022) 国家重点研发计划(2016YFD0500201) 泰山学者工程专项经费等资助
【分类号】：S852.651

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