番鸭呼肠孤病毒感染诱导细胞自噬的研究

发布时间：2018-05-13 07:11

本文选题：番鸭呼肠孤病毒 + 细胞自噬　；参考：《病毒学报》2017年02期

【摘要】：本研究通过透射电镜观察自噬体的形态学结构,蛋白免疫印迹(Western blot)检测细胞自噬相关蛋白LC3II和磷酸化mTOR(p-mTOR)表达量的动态变化趋势,探索番鸭呼肠孤病毒(MDRV)感染对细胞自噬的影响。结果显示,MDRV-YB株感染的DF-1细胞中可见典型的包裹胞浆成分的双层膜结构;MDRV感染番鸭成纤维细胞(MDFs)和DF-1细胞后0~60h内,其LC3II表达量均呈现先升高后降低的趋势,且均在感染后36h达到最大值,其p-mTOR表达量均呈现先降低后回升的趋势,且分别在感染后36h和24h达到最低值;灭活MDRV组细胞未见LC3II表达。结果表明MDRV能够诱导感染的MDFs和DF-1细胞持续较长时间细胞自噬,而灭活的MDRV不能引起细胞自噬。
[Abstract]:In this study, the morphological structure of autophagy was observed by transmission electron microscope, and the expression of autophagy associated protein (LC3II) and phosphorylated mTOR-p-mTOR-mTor were detected by Western blot. The effect of muscovy duck reovirus (MDRV) infection on autophagy was investigated. The results showed that the expression of LC3II in DF-1 cells infected with MDRV-YB strain showed a tendency of increasing first and then decreasing within 60 h after infection with MDRV-YB strain and MDRV-infected muscovy duck fibroblasts (MDFs) and DF-1 cells. The p-mTOR expression reached the highest value at 36h after infection, and the expression of p-mTOR decreased first and then increased, and reached the lowest value at 36h and 24h after infection, and no LC3II expression was found in MDRV group. The results showed that MDRV could induce long-term autophagy of infected MDFs and DF-1 cells, while inactivated MDRV could not induce autophagy.
【作者单位】：福建农林大学动物科学学院;福建省兽医中药与动物保健重点实验室;
【基金】：国家自然科学基金,项目编号(u1305212),题目:闽台特色水禽重要病毒的致病机理研究;国家自然科学基金,项目编号(31372474),题目:猴头菇多糖对MDRV致番鸭肠黏膜免疫功能损伤的修复及其机制研究
【分类号】：S858.32

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