大熊猫源石膏样小孢子菌培养条件的筛选

发布时间：2018-05-15 01:27

  本文选题：大熊猫 + 石膏样小孢子菌　； 参考：《湖南农业大学学报(自然科学版)》2017年02期

【摘要】：为探明大熊猫源石膏样小孢子菌的最佳培养条件,在不同培养基、碳源、氮源、温度、pH等条件下培养石膏样小孢子菌,用十字交叉法测量菌落每天的生长情况。结果显示,石膏样小孢子菌在沙氏葡萄糖蛋白胨琼脂培养基上生长最好,在改良沙氏葡萄糖蛋白胨琼脂培养基、马铃薯葡萄糖琼脂培养基、高盐培养基上均生长良好,但在皮肤癣菌鉴定培养基和子囊孢子培养基上生长缓慢;5种碳源对菌株生长的影响不大,其中葡萄糖使该菌生长较好;在5种氮源培养基中,除了在以尿素为氮源的培养基中该菌株不能生长外,在以蛋白胨、硝酸钠、草酸铵、硫酸铵为碳源的培养基中该菌均能生长,且在以蛋白胨为氮源的培养基中生长最好;该菌株生长的最适培养温度为25℃,在15~35℃均可生长,在35℃时生长最差;在pH值为10时生长最好,在pH值为5、6、8、9时均能够良好生长,比在pH值为7时的生长更好,在pH值为4、11时的生长较差。
[Abstract]:In order to find out the best culture conditions of Gypsum microspore from giant panda, microsporum gypsum was cultured in different culture medium, carbon source, nitrogen source, temperature and pH, and the growth of colony was measured by cross method. The results showed that the microsporozoa gypsum grew best on the salsa glucose peptone Agar medium, and on the modified Salvia glucose peptone Agar medium, the potato glucose Agar medium and the high salt medium. However, the effects of five carbon sources on the growth of Trichophyton dermatophytes and ascospore medium were not significant, and glucose made the strain grow better. Except that the strain could not grow in the medium with urea as nitrogen source, the strain could grow in the medium with peptone, sodium nitrate, ammonium oxalate and ammonium sulfate as carbon source, and the best growth was in the medium with peptone as nitrogen source. The optimum growth temperature of the strain is 25 鈩，

本文编号：1890370