广东小鼠腺病毒血清学调查及病毒和抗体在人工感染小鼠体内消长规律研究

发布时间：2018-05-15 02:10

本文选题：小鼠腺病毒 + 血清学　；参考：《中国比较医学杂志》2017年01期

【摘要】：目的了解我省小鼠腺病毒(Mad)自然感染情况及探究人工感染Mad小鼠体内各脏器组织中病毒分布规律及血清抗体变化。方法采用ELISA方法对2007年和2015年饲养于普通环境小鼠血清及2013年~2015年广东省12家监督单位和32家委托单位SPF级小鼠血清进行病毒抗体检测。利用腹腔注射方法感染36只3周龄BALB/c小鼠,0.2 m L/只,浓度为4.5×106copies/μL,每天观察动物临床表现,并于攻毒前第0天和攻毒后第3、7、10、15、18、21、30、37、44、51、60天剖检小鼠(各3只),取组织标本(心、肝、脾、肺、肾、脑、胃、盲肠内容物)及血清,应用实时荧光定量PCR(QPCR)方法检测组织病毒核酸,ELISA方法检测血清抗体水平。结果普通饲养小鼠抗体阳性率为24.44%~84.15%,其中以Mad-2型K87株血清型为主。SPF级小鼠Mad血清抗体阳性率均为0%。所有攻毒小鼠均呈隐性感染,无临床表现。攻毒后第3天及7天小鼠各组织病毒核酸检测阳性率均为100%(3/3),其中以脾脏100%阳性率维持时间最长(60 d)。除肝脏外,小鼠各组织病毒含量均在攻毒后7 d达到高峰,其中以脾脏病毒含量最高(5.5×105copies/μL),其次是心脏(3.4×105copies/μL)、盲肠内容物(2.6×105copies/μL)和胃(2.6×105copies/μL),脑为0.8×105copies/μL。攻毒后15 d可测出血清抗体,在37 d达到峰值,此后至60 d一直维持高水平。结论 SPF级小鼠Mad感染率低,普通环境饲养小鼠Mad感染率高。人工感染小鼠脾脏病毒含量最高,阳性率维持时间最长,说明病毒可以在脾脏内长期复制,感染后第7天为组织病毒核酸最佳检测时间点。血清抗体在感染后15~60 d内均可以作为监测指标。
[Abstract]:Objective to investigate the natural infection of adenovirus Mada in mice and to explore the distribution of virus and the changes of serum antibodies in various organs of artificially infected Mad mice. Methods ELISA method was used to detect the virus antibodies in the sera of mice raised in general environment in 2007 and 2015, and the sera of 12 supervising units and 32 commissioned units in Guangdong Province from 2013 to 2015. 36 3-week-old BALB/c mice were infected by intraperitoneal injection with a concentration of 4.5 脳 106copies/ 渭 L. The clinical manifestations of the mice were observed every day. The mice were dissected on the 0 th day before the attack and the 3th day after the attack. The mice were dissected (3 mice each) for tissue specimens (heart, liver, spleen, lung, lung). Kidney, brain, stomach and cecum contents) and serum were detected by real-time fluorescence quantitative PCR- QPCR- Elisa method. Results the positive rate of antibody was 24.44% and 84.15% respectively in common bred mice. The positive rate of antibody in Mad serum of Mad-2 K87 strain was 0. All the mice were infected with recessive infection, and no clinical manifestations were found. The positive rate of virus nucleic acid detection in each tissue of mice on the 3rd and 7th day after the attack was 100 / 3 / 3 / 3 respectively, and the spleen 100% positive rate lasted for the longest time of 60 days. With the exception of the liver, the virus content of each tissue reached its peak at 7 days after the attack, in which the highest virus content in spleen was 5.5 脳 105copies/ 渭 L, followed by cardiac virus content of 3.4 脳 105copies/ 渭 L, caecum content of 2.6 脳 105copies/ 渭 L) and stomach tissue of 2.6 脳 105copies/ 渭 L, brain of 0.8 脳 105copies/ 渭 L. The serum antibody was detected 15 days after the attack and reached the peak at 37 days, and maintained a high level until 60 days later. Conclusion the infection rate of Mad in SPF grade mice is lower than that in common environment mice. The spleen virus content of artificially infected mice was the highest, and the positive rate was the longest, which indicated that the virus could be replicated in the spleen for a long time, and the best time point for the detection of tissue virus nucleic acid was on the 7th day after infection. The serum antibody can be used as a monitoring index within 1560 days after infection.
【作者单位】：广东省实验动物监测所;广东省实验动物重点实验室;
【基金】：广东省科技计划项目(2014B070706006)
【分类号】：S858.91

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