产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制

发布时间：2018-05-15 02:30

本文选题：大肠杆菌K + 仔猪　；参考：《中国畜牧兽医》2017年01期

【摘要】：为探讨产肠毒素大肠杆菌(ETEC)K88感染仔猪发生炎症反应的分子机制,试验用ETEC K88灌服断奶仔猪,ELISA法检测攻毒后仔猪血清中白细胞介素8(IL-8)含量,实时荧光定量PCR方法检测淋巴结中Toll样受体2(TLR2)、Toll样受体4(TLR4)及其信号通路相关基因(髓样分化因子88(MyD88)、Toll相互作用蛋白(Tollip)、B细胞淋巴瘤因子3(Bcl3))的mRNA相对表达水平。结果发现,仔猪攻毒ETEC K88后6和24h血清IL-8含量和淋巴结TLR2/4的表达水平均极显著或显著高于对照组(P0.01;P0.05),且感染后24h显著低于感染后6h(P0.05);仔猪感染ETEC K88后24h淋巴结中MyD88、Tollip和Bcl3的表达水平均极显著高于对照组(P0.01),但是感染后6h时与对照组相比均无显著差异(P0.05)。综上所述,ETEC K88感染仔猪可能是通过TLR2/4-MyD88信号通路产生炎症因子IL-8,促使仔猪出现炎症反应,且该炎症反应可能受Tollip和Bcl3蛋白的调控而被减弱。
[Abstract]:In order to investigate the molecular mechanism of inflammatory reaction in piglets infected with enterotoxigenic Escherichia coli (ETEC) K88, the serum interleukin-8 (IL-8) was detected by ETEC K88 in weaned piglets by Elisa. Real-time quantitative PCR was used to detect the relative expression of Toll like receptor 2TLR2TLR4) and its signal transduction related gene (myeloid differentiation factor 88MyD88Toll interaction protein Tollipo B cell lymphoma factor 3 Bcl3) in lymph nodes. And it turns out, The levels of serum IL-8 and lymph node TLR2/4 expression in piglets at 6 and 24 hours after infection with ETEC K88 were extremely significant or significantly higher than those in the control group, and were significantly lower at 24 h after infection than those at 6 h after infection, and the expression of MyD88 Tollip and Bcl3 in lymph nodes 24 hours after ETEC K88 infection in piglets were significantly lower than those in the control group. The average value was significantly higher than that of the control group (P 0.01), but there was no significant difference between the control group and the control group at 6 h after infection. In conclusion, the inflammatory factor IL-8 may be produced by the TLR2/4-MyD88 signaling pathway in the piglets infected with ETEC K88, and the inflammatory response may be weakened by the regulation of Tollip and Bcl3 proteins.
【作者单位】：天津市畜牧兽医研究所;天津市畜禽健康养殖技术工程中心;
【基金】：国家自然科学基金(31402087)
【分类号】：S858.28

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