镉及α-硫辛酸对大鼠肝脏GJIC功能的影响

发布时间：2018-05-30 00:24

本文选题：镉 + α-硫辛酸　；参考：《中国兽医学报》2017年11期

【摘要】：为探讨镉及α-硫辛酸(α-LA)对大鼠肝细胞缝隙通讯功能(GJIC)的影响,将30只100g左右SD雌性大鼠随机均分为3组:对照组(自由饮水及超纯水灌胃)、镉组(50mg/L醋酸镉自由饮水及超纯水灌胃)、镉与α-LA共同处理组(50mg/L醋酸镉自由饮水及α-LA灌胃50mg/kg)。12周后,采用荧光染料标记传输示踪法(IL/DT)测定GJIC功能,免疫印迹检测大鼠肝脏中Cx43、p-Cx43、Cx32蛋白表达变化,荧光定量PCR法检测大鼠肝脏中Cx32、Cx26、Cx43mRNA表达量的变化。IL/DT结果显示,大鼠长时间饮用醋酸镉后,肝脏细胞间荧光黄两侧扩散距离极显著减少(P0.01),α-LA处理后极显著提高(P0.01)。肝脏中Cx43、p-Cx43、Cx32蛋白表达量呈显著或极显著的降低,Cx43、Cx32、Cx26mRNA转录基因表达也呈显著或极显著的降低(P0.05或P0.01),α-LA处理后都有显著或极显著升高(P0.05或P0.01)。结果表明:长期饮用醋酸镉可以抑制大鼠肝细胞GJIC,可能是连接蛋白或基因表达减少,α-LA能缓解镉对GJIC的影响。
[Abstract]:To investigate the effects of cadmium and 伪 -lipoic acid (伪 -LAA) on gap communication function of rat hepatocytes, Thirty female Sprague-Dawley rats were randomly divided into three groups: control group (free drinking water and ultra-pure water), cadmium acetate free drinking water (50 mg / L) in cadmium group and ultra-pure water, cadmium acetate free drinking water and 伪 -LA treatment group (50 mg / L CD _ 2O _ 3 free drinking water and 伪 -LA) after 50mg/kg).12 week. The function of GJIC was detected by fluorescent dye transfer tracer method. Western blot was used to detect the expression of Cx43 p-Cx43 Cx32 protein in rat liver. The results of IL / DT showed that the expression of Cx32Cx26Cx43 mRNA in rat liver was detected by fluorescence quantitative PCR, and the results of IL / DT showed that after drinking cadmium acetate for a long time, the expression of Cx43 protein in rat liver was detected by fluorescence quantitative PCR. The diffusion distance between the two sides of the liver cells decreased significantly, and that of 伪 -LA increased significantly. In liver, the expression of Cx43, p-Cx43, Cx32 was significantly or extremely significantly decreased, and the expression of Cx43, Cx32, Cx26 mRNA was significantly or extremely significantly decreased by P0.01or P0.01. after 伪 -LA treatment, there was a significant or extremely significant increase in the mRNA expression of Cx43, Cx32 and Cx26, respectively (P0.05 or P0.01A) after treatment with 伪 -LA, there was a significant or extremely significant increase in the expression of Cx43, Cx32 and Cx26 mRNA, respectively. The results showed that drinking cadmium acetate for a long time could inhibit the expression of connexin or gene in rat hepatocytes, and 伪 -LA could alleviate the effect of cadmium on GJIC.
【作者单位】：扬州大学兽医学院;扬州市广陵区兽医站;
【基金】：江苏高校优势学科建设工程项目江苏省动物重要疫病与人兽共患病防控协同创新中心项目
【分类号】：S859.8

【相似文献】