河南某鸡场地方品种鸡禽白血病病毒感染状态检测

发布时间：2018-05-30 00:37

本文选题：地方品种鸡 + 禽白血病　；参考：《西北农业学报》2017年03期

【摘要】：为了解河南某鸡场地方品种鸡禽白血病病毒(Avian Leukosis Virus,ALV)感染状态,采集该鸡场绿壳蛋鸡、青脚麻鸡和固始鸡的泄殖腔棉拭子、蛋清和血清,利用ELISA方法检测泄殖腔棉拭子、蛋清和血清样品中的ALV-p27抗原及血清样品中ALV-A/B和ALV-J抗体的水平,同时采集其血液接种DF-1细胞进行外源性ALV的分离鉴定。结果表明,绿壳蛋鸡泄殖腔棉拭子、蛋清和血清中的ALV-p27抗原阳性率分别为23.55%、11.96%和26.63%,血清中ALV-A/B和ALV-J抗体的阳性率分别为30.43%和16.85%,外源性ALV的分离阳性率为13.04%;青脚麻鸡泄殖腔棉拭子、蛋清和血清中抗原阳性率分别为10.99%、5.43%和22.83%,血清中ALV-A/B和ALV-J抗体的阳性率分别为18.48%和6.52%,外源性ALV的分离阳性率为8.00%;固始鸡泄殖腔棉拭子、蛋清和血清中抗原阳性率分别为21.07%、12.32%和25.00%,血清中ALVA/B和ALV-J抗体的阳性率分别为28.26%和17.39%,外源性ALV的分离阳性率为12.50%。表明3个地方品种鸡都不同程度地感染外源性ALV,且可能存在ALV-A/B和ALV-J 2种或3种亚群同时感染。
[Abstract]:In order to understand the infection status of avian Leukosis virus (Avian Leukosis virus) infection in a local breed of chicken farm in Henan Province, the cloacal cotton swabs, egg white and serum of the green shell laying hens, green legged hens and Gushi hens were collected. The cloacal cotton swabs were detected by ELISA method. The levels of ALV-p27 antigen in egg white and serum samples and ALV-A/B and ALV-J antibodies in serum samples were collected and their blood was inoculated with DF-1 cells for isolation and identification of exogenous ALV. The results showed that the positive rates of ALV-p27 antigen in cloacal cotton swab, egg white and serum of green shell laying hens were 23.55, 11.96% and 26.63%, respectively, the positive rates of ALV-A/B and ALV-J antibody in serum were 30.43% and 16.85%, the positive rate of exogenous ALV was 13.04%, the positive rate of cloacal cotton swab was 13.04%. The positive rates of antigen in egg white and serum were 10.99% and 22.83%, the positive rates of ALV-A/B and ALV-J antibody in serum were 18.48% and 6.52%, the positive rate of exogenous ALV was 8.00%, the positive rate of cloacal cotton swab in Gushi chicken was 8.00%. The positive rates of antigens in egg white and serum were 21.07% and 25.00%, the positive rates of ALVA/B and ALV-J antibodies in serum were 28.26% and 17.39%, and the positive rates of extrinsic ALV were 12.50% and 12.50%, respectively. The results showed that all of the three local breeds were infected with exogenous ALV to varying degrees, and two or three subpopulations of ALV-A/B and ALV-J might be infected simultaneously.
【作者单位】：河南牧业经济学院动物科技学院;
【基金】：河南省科技攻关计划(152102110089) 校科技创新团队项目(HUAHE2015003)~~
【分类号】：S858.31

【相似文献】