大雁、斑头雁和天鹅源沙门菌的耐药性分析

发布时间：2018-05-30 00:41

本文选题：沙门菌 + 耐药性　；参考：《动物医学进展》2017年09期

【摘要】：研究大雁、斑头雁和天鹅源沙门菌的耐药性及其耐药基因的携带情况。采用K-B纸片法对33株大雁、斑头雁和天鹅源沙门菌进行20种抗菌药物敏感性测定,应用PCR对12种耐药基因的携带情况进行检测。结果表明,33株沙门菌分离株对阿莫西林、萘啶酸耐药性最高,分别为93.94%、87.88%,对氨苄西林(54.55%)、四环素(48.49%)、多西环素(48.49%)、卡那霉素(60.61%)、庆大霉素(48.49%)、链霉素(66.67%)耐药性比较严重,对氧氟沙星、培氟沙星、诺氟沙星敏感。共检测到9种耐药基因,其中blaCMY-2阳性率为87.88%,qnr、aac(6′)-Ib-cr阳性率分别为17.24%、86.21%,tetA、tetB阳性率分别为45%、20%,aph(3)Ⅱ、aac(3)Ⅱ、aadA1阳性率分别为100%、10%、20%,sul2阳性率为100%,未检出blaTEM-1、tetC、tetG基因。说明大雁、斑头雁和天鹅源沙门菌严重耐药,且携带多种耐药基因。
[Abstract]:To study the drug resistance and drug resistance of Salmonella spp. K-B disk method was used to detect the sensitivity of 20 antimicrobial agents to 33 strains of wild geese, wild goose and Salmonella swan, and PCR was used to detect the carrying of 12 drug resistant genes. The results showed that 33 strains of salmonella had the highest resistance to amoxicillin and nalididinic acid, which were 93.9447.88, 54.5550, 48.49cm, 48.49m, 48.49m, 60.61m, 48.49m, 48.49m, 48.49m, 48.49m, 48.49m, 48.49m, 48.49m, 48.49m, 48.49m, 66.67%, respectively, to ampicillin, to ofloxacin, and to ofloxacin. Pefloxacin is sensitive to norfloxacin. A total of 9 resistant genes were detected, of which the positive rate of blaCMY-2 was 87.88 and the positive rate of blaCMY-2 was 17.2446.21 and 86.21, respectively. The positive rate of blaCMY-2 was 450.The positive rate of aadA1 was 100%. The positive rate of blaTEM-1tetCtetG was not detected. The results showed that wild goose, wild goose and swan source Salmonella were highly resistant to drugs and carried many drug resistance genes.
【作者单位】：西南民族大学生命科学与技术学院;
【基金】：四川省科技厅应用基础研究项目(2013JY0045)
【分类号】：S852.61

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