秦岭部分珍稀野生动物芽囊原虫感染情况及种群结构研究

发布时间：2018-06-06 11:58

本文选题：芽囊原虫 + 珍稀珍稀动物　；参考：《西北农林科技大学》2017年硕士论文

【摘要】：芽囊原虫(Blastocystis sp.)是一种广泛分布的寄生于肠道的原虫,在欠发达地区的更为流行,引起腹痛腹泻等消化系统症状,对人的健康和动物的生长发育具有不良影响。芽囊原虫的宿主广泛,可感染人和其他哺乳动物及鸟类。近期的研究发现,珍稀野生动物在人和动物的芽囊原虫感染过程中具有公共卫生隐患。因此,掌握芽囊原虫的感染情况和种群结构对评估其人兽共患潜能、防控宿主及其具有重要意义。秦岭地区珍稀野生动物资源独特而丰富,本研究通过基于SSUrRNA基因序列的分子检测对该地区两个野生动物饲养点部分珍稀野生动物进行芽囊原虫感染情况进行调查,并对获得的阳性分离株进行种群结构分析。获得以下结果:1.2015年6月至2016年1月,对秦岭地区37个物种共497份粪便样品进行芽囊原虫分子检测,阳性样品PCR扩增结果为1,100bp的基因片段。7个动物类群总感染率为40.2%(200/497),其中非人灵长类(75.9%)和有袋类(72.7%)感染率最高,偶蹄类(56.3%)和单蹄类(22.2%)次之,鸟类感染率(2.2%)最低。象类和食肉类为芽囊原虫阴性。世界范围内首次在果下马、蒙古野驴、羚牛、羊驼、大羚羊、梅花鹿、马鹿、列氏水羚、牦牛和白唇鹿中发现芽囊原虫。两个采样点感染情况一致,均有季节性变化——夏季到冬季感染率逐渐下降。2.基于芽囊原虫阳性样品的SSUrRNA基因的种系进化关系分析,本研究共发现了13种亚型,包括8种已有亚型(亚型1~3,5,10和12~14)和5种可能的新亚型(暂时命名为亚型18~22)。在已有亚型中,亚型13和5具有人兽共患潜能;可能的新亚型中,亚型19和20可能具有人兽共患潜能。亚型10是优势亚型(38.5%),分布于17种野生动物,亚型5首次在鸵鸟内发现。
[Abstract]:Blastocystis sp. It is a widely distributed protozoa, which is more prevalent in underdeveloped areas, causing abdominal pain, diarrhea and other digestive system symptoms, which has a negative impact on human health and the growth and development of animals. The protozoa has a wide range of hosts and can infect humans and other mammals and birds. Recent studies have found that rare wild animals have a public health hazard in the process of infection of human and animal bud sac protozoa. Therefore, it is important to know the infection status and population structure of Bud cyst protozoa in order to evaluate its zoonotic potential and control its host. Rare and rare wildlife resources in Qinling region are unique and abundant. In this study, the infection of bud cyst protozoa of some rare wild animals in two wild animal breeding sites in this area was investigated by molecular detection based on SSUrRNA gene sequence. The population structure of the positive isolates was analyzed. The following results were obtained: 1. From June 2015 to January 2016, 497 fecal samples from 37 species in Qinling region were detected for protozoa sprouting. The total infection rate of the seven animal groups was 40.22 / 497, including 75.9% of the non-human primate and 72.7% of the marsupial.) the infection rate was the highest, followed by 56.3) and 22.2b), and the infection rate of birds was the lowest. The herbivorous meat was negative for Bud cyst protozoa. Bud sac protozoa was first found in fruit dismount, Mongolian wild donkey, antelope, alpaca, large antelope, sika deer, Wapiti, Lees gazelle, yak and white-lipped deer in the world. The infection rate in the two sampling sites was the same, and there was seasonal variation-the infection rate decreased gradually from summer to winter. Based on phylogenetic analysis of SSUrRNA gene in protozoa positive samples, a total of 13 subtypes were identified in this study, including 8 existing subtypes (subtypes 1, 3, 5, 10 and 12, 14) and 5 possible new subtypes (temporarily named as subtype 18t22). Among the existing subtypes, subtypes 13 and 5 have zoonotic potential, and subtypes 19 and 20 may have zoonotic potential among the possible new subtypes. Subtype 10 is a dominant subtype 38.5, distributed in 17 species of wild animals. Subtype 5 was first found in ostrich.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.9

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