口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征3种疫苗同步两点免疫的田间应用试验

发布时间：2018-06-09 03:19

本文选题：口蹄疫 + 猪瘟　；参考：《中国畜牧兽医》2017年08期

【摘要】：为探索农村散养猪口蹄疫(foot-and-mouth disease,FMD)、猪瘟(classical swine fever,CSF)、高致病性猪繁殖与呼吸综合征(highly pathogenic porcine reproductive and respiratory syndrome,HP-PRRS))疫苗的最佳免疫程序,在2个镇开展田间免疫试验,A镇采用"332法"免疫程序,即先用FMD和CSF疫苗同时在颈部分两侧注射,间隔14d后再用HP-PRRS疫苗注射;B镇采用"321法"免疫程序,即将CSF和HP-PRRS疫苗混合后作一针在颈部一侧注射,FMD疫苗作一针在颈部另一侧注射。应用ELISA方法检测FMDV、CSFV和HP-PRRSV抗体,应用RT-PCR方法检测FMDV、CSFV和HP-PRRSV病原;统计、分析生猪免疫密度、免疫副反应率、生猪死淘率、散养猪抗体阳性率、屠宰猪抗体阳性率、死淘猪病原阳性率、屠宰猪病原阳性率等指标。结果显示,应用"321法"的B镇与应用"332法"的A镇相比,其HP-PRRS免疫密度、散养猪CSFV和HP-PRRSV抗体阳性率、屠宰猪HPPRRSV抗体阳性率均显著增加(P0.05),散养猪死淘率、HP-PRRSV阳性率均显著下降(P0.05),而其他主要考核指标则差异不显著(P0.05)。以上结果表明,应用"321法"免疫程序的免疫效果优于"332法",值得在临床推广应用。
[Abstract]:In order to explore the foot-and-mouth disease (FMD), classical swine fever, CSF, and high pathogenic porcine reproductive and respiratory syndrome (highly pathogenic porcine reproductive and)) the best immunization program was carried out in 2 towns, and the "332 method" immunization course was adopted in the town. Order, first injection of FMD and CSF vaccine on both sides of the neck and then injected with HP-PRRS vaccine at intervals of 14d; B town adopted a "321 method" immunization program, followed by a combination of CSF and HP-PRRS vaccines for injection on the neck side, and FMD vaccine as a needle on the other side of the neck. FMDV, CSFV and HP-PRRSV antibodies should be used to detect FMDV, CSFV and HP-PRRSV antibodies. Methods to detect the pathogens of FMDV, CSFV and HP-PRRSV, and to analyze the immune density of the pig, the rate of side reaction, the rate of pig death, the positive rate of pig antibody, the positive rate of the slaughtered pig antibody, the positive rate of the dead pig and the positive rate of the slaughtered pig, and the results showed that the HP-PRRS immunization of the "321 methods" of the B town was compared with the "332 method" of A town. The positive rate of CSFV and HP-PRRSV antibody of pig, the positive rate of HPPRRSV antibody in slaughtered pig increased significantly (P0.05), the rate of pig death naughty and HP-PRRSV positive rate decreased significantly (P0.05), while the other main assessment indexes were not significant (P0.05). The results showed that the immunization effect of the "321 method" immunization program was better than the "332 method", and it was worth in the future. Bed popularization and application.
【作者单位】：百色市动物疫病预防控制中心;平果县动物疫病预防控制中心;百色农业学校;田东县动物疫病预防控制中心;广西动物疫病预防控制中心;
【基金】：广西科学研究与技术开发计划项目(桂科转14125004-22) 广西水产畜牧科技项目(桂渔牧科201528017)
【分类号】：S858.28

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