黄柏水提物联合抗菌药对产ESBLs大肠杆菌的抑菌效果

发布时间：2018-06-09 06:15

本文选题：黄柏 + 抗菌药　；参考：《南方农业学报》2016年03期

【摘要】：【目的】探讨黄柏水提物与抗菌药联用对产超广谱β-内酰胺酶(ESBLs)大肠杆菌的抑制效果,为黄柏的综合开发利用提供参考依据。【方法】采用二倍微量肉汤稀释法测定黄柏水提物与抗菌药的最小抑菌浓度(MIC),并采用微量棋盘稀释法测定黄柏水提物与抗菌药联用后的分级抑菌浓度指数(FICI)。【结果】黄柏水提物对产ESBLs大肠杆菌的MIC为500 mg/m L,黄柏水提物与头孢曲松钠、阿莫西林、痢菌净、磺胺间甲氧嘧啶、头孢噻呋钠、头孢他啶、氟苯尼考、环丙沙星、诺氟沙星、左旋氧氟沙星、克林沙星、加替沙星、头孢噻肟联用后的FICI为0.50~2.01,其中,黄柏水提物与阿莫西林、头孢曲松钠、头孢噻呋钠、头孢他啶、头孢噻肟、痢菌净联用存在相加作用,与氟苯尼考联用存在协同作用,与诺氟沙星、左旋氧氟沙星、克林沙星、磺胺间甲氧嘧啶间为无关作用,与环丙沙星、加替沙星联用呈拮抗作用。【结论】黄柏水提物与青霉素类、第三代头孢菌素、痢菌净、氟苯尼考联用是通过相加或协同作用提高药物对产ESBLs大肠杆菌的抑制效果,但不宜与喹诺酮类抗菌药联合用药。
[Abstract]:[objective] to study the inhibitory effect of water extract of Phellodendron chinensis and antimicrobial agents on extended-spectrum 尾 -lactamase (ESBLs) -producing Escherichia coli. [methods] the minimal inhibitory concentration (MIC) of aqueous extract and antimicrobial agents of Phellodendron chinensis was determined by double microamount broth dilution method, and the water extract and resistance of Phellodendron chinensis were determined by chessboard dilution method. [results] the MIC of ESBLs-producing Escherichia coli (ESBLs-producing Escherichia coli) was 500 mg/m / L, water extract and ceftriaxone sodium, respectively. Amoxicillin, sulfamethoxine, cefotaxime sodium, ceftazidime, florfenicol, ciprofloxacin, norfloxacin, levofloxacin, clinfloxacin, gatifloxacin, cefotaxime were 0.502.01 of which, The water extract of Phellodendron chinensis has additive effect with amoxicillin, ceftriaxone sodium, ceftiofuroxime sodium, ceftazidime, cefotaxime, furalum, synergism with florfenicol, norfloxacin, levofloxacin, clinfloxacin, Sulfamethoxine was independent of sulfadiazine, and antagonistic with ciprofloxacin and gatifloxacin. [conclusion] the aqueous extract of Phellodendron chinensis is associated with penicillin, third generation cephalosporin, dysentery. The combination of florfenicol and quinolones can improve the inhibition of ESBLs-producing Escherichia coli by additive or synergistic effect, but it is not suitable to combine with quinolones.
【作者单位】：广西大学动物科学技术学院;
【基金】：国家自然科学基金项目(31460675) 国家科技支撑计划项目(2012BAD25B04) 广西自然科学基金项目(2013GXNSFAA019070) 广西科学研究与技术开发计划项目(0992014-2)
【分类号】：S853.7

【相似文献】