蒙古羊限饲及补偿对空肠和结肠及相关实质器官的影响

发布时间：2018-06-09 15:11

本文选题：肝脏 + 脾脏　；参考：《内蒙古农业大学》2017年硕士论文

【摘要】：本论文旨在研究限饲及不同方式补饲对蒙古公羔羊肝、脾、胰腺、结肠的形态结构及空肠和肝脏相关基因表达的影响。选用30只四月龄蒙古公羔羊,平均体重为20.70±3.51kg,随机平均分为对照组(CG)和限制组(RG)。试验期分限制饲养(0-60d)和补偿饲养(61-120d)。限制期结束,从两组中随机选五只屠宰采样。每组剩余随机分成两组,青干草组(CH、RH)和秸秆组(CM、RM),青干草组饲喂精粗比为5:5的青干草精料日粮,秸秆组饲喂精粗比3:7的秸秆精料日粮,补偿期结束后,全部屠宰采样。采用组织切片法研究肝、脾、胰腺、结肠形态结构变化,对空肠及肝脏进行实时荧光定量PCR检测代谢相关基因变化,从而对限制和补偿生长对羊空肠结肠及相关实质器官的影响做进一步研究。结果表明:试验一结果:(1)本试验条件下蒙古公羔羊结肠的结果:结肠黏膜层代谢旺盛,营养限制—段时间,由于动物机体趋于吸收更多能量,黏膜层厚度代偿性地升高。(2)本试验条件下蒙古公羔羊肝脏的结果:通过60天限制饲养,RG组肝细胞周长显著低于CG组。而60天营养补偿后,肝脏细胞周长恢复到正常水平。肝脏中央静脉周长、肝细胞核周长恢复正常。(3)本试验条件下蒙古公羔羊脾脏的结果:营养限制与补偿对脾脏白髓影响不明显,可能当发生免疫应答反应后脾白髓会出现显著的增大。(4)本试验条件下蒙古公羔羊胰腺的结果:胰腺腺泡周长、胰腺灰度值,营养限制期与补偿期后变化不显著。试验二结果:(1)空肠基因表达的变化:本试验条件下,高精料补偿有助于Claudin-1基因mRNA表达,提高了细胞屏障作用。营养限制60天,限饲组Caveolin-1基因mRNA表达量高于对照组3.27倍。营养补偿60天后,可能由于Caveolin-1基因在限饲后基因表达代偿性显著升高,而补偿后恢复到正常水平。营养限制与补偿对空肠connexin-43基因的表达没有影响。(2)肝脏脂肪代谢基因的变化:营养限制后PPARγγ基因mRNA表达显著高于对照组,营养限制导致脂肪分解,肝脏合成甘油三酯进行储存。营养限制后FASN和ACACA基因的表达量显著降低,主要原因是由于乙酸的降低,高精料没有促进FASN和ACACA基因的表达显著升高。
[Abstract]:The purpose of this study was to investigate the effects of dietary restriction and supplementation on the morphological structure of liver, spleen, pancreas and colon and the expression of genes related to jejunum and liver in Mongolian male lambs. Thirty four-month-old Mongolian male lambs with an average weight of 20.70 卤3.51 kg were randomly divided into control group (CGG) and restriction group (RGG). During the trial period, the feeding period was limited to 0-60 days) and the compensatory feeding time was 61-120 days. At the end of the restriction period, five slaughter samples were randomly selected from the two groups. Each group was randomly divided into two groups: the green hay group (CHRH) and the straw group (CMR RMN), the green hay group fed with the concentrate diet of the crude ratio of 5:5 and the straw group fed with the concentrate ration of the concentrate at 3:7. After the compensation period was over, all the samples were slaughtered. The morphologic and structural changes of liver, spleen, pancreas and colon were studied by tissue section method. The changes of metabolism-related genes in jejunum and liver were detected by real-time fluorescence quantitative PCR. The effects of limiting and compensating growth on jejunal colon and related parenchymal organs were further studied. The results showed that under the condition of this experiment, the colon of Mongolian male lambs was metabolized vigorously, nutrition was limited for a period of time, and the animal body tended to absorb more energy. The results of the experiment showed that the liver circumference of Mongolian male lambs was significantly lower than that of CG group after 60 days of restriction. However, after 60 days of nutrition compensation, the peripheral length of liver cells returned to normal level. The results of this study were as follows: nutrition restriction and compensation had no significant effect on white pulp of spleen. The results of this study were as follows: the acinar circumference of the pancreas, the gray value of the pancreas, the nutritional limitation period and the compensation period had no significant changes. The results of experiment 2: the change of gene expression in jejunum: under this condition, high concentrate compensation was helpful to Claudin-1 gene mRNA expression and enhanced cell barrier. The mRNA expression of Caveolin-1 gene was 3.27 times higher in diet restriction group than that in control group for 60 days. After 60 days of nutrition compensation, Caveolin-1 gene expression increased significantly after feeding restriction, but returned to normal level after compensation. Nutrition restriction and compensation had no effect on the expression of connexin-43 gene in jejunum. After nutrition restriction, the expression of FASN and ACACA genes decreased significantly, mainly due to the decrease of acetic acid, and the high concentrate did not promote the expression of FASN and ACACA genes.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S826

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