山羊FSHR基因编码蛋白的结构及功能分析

发布时间：2018-06-26 22:38

本文选题：FSHR基因 + 系统发育分析　；参考：《河南农业科学》2017年07期

【摘要】：通过Prot Param和ProtScale等在线软件对山羊FSHR基因编码蛋白的理化性质、亚细胞定位及功能进行分析,分别使用Chou-Fasman和同源建模方法对二级、三级结构进行预测,并对采用邻近法构建的不同物种FSHR基因编码蛋白的系统发育树进行分析。结果显示,山羊FSHR基因编码蛋白由695个氨基酸残基组成,包含7个跨膜域和1个G蛋白耦合受体,属于不稳定蛋白,具有较高的亲水性;亚细胞定位结果显示,FSHR基因编码蛋白分布在内质网、液泡和线粒体内;二级结构主要是α螺旋,三级结构富含helix螺旋结构域和长链卷曲。对不同物种FSHR基因编码蛋白进行系统发育分析发现,与其他物种相比,山羊与绵羊、家牛、野猪亲缘关系较近,系统发育分析结果与其动物学分类结果一致。
[Abstract]:The physicochemical properties, subcellular localization and function of goat FSHR gene coding protein were analyzed by Prot Param and ProtScale online software. Chou-Fasman and homology modeling methods were used to predict the secondary and tertiary structures, respectively. The phylogenetic tree of FSHR gene encoding proteins of different species was constructed by proximity method. The results showed that the goat FSHR gene encoding protein consists of 695 amino acid residues, including 7 transmembrane domains and 1 G protein coupled receptor, which belongs to unstable protein and has high hydrophilicity. The results of subcellular localization showed that the protein encoding FSHR gene was distributed in endoplasmic reticulum, vacuole and mitochondria, the secondary structure was mainly 伪 helix, and the tertiary structure was rich in helix domain and long chain crimp. Phylogenetic analysis of FSHR gene encoding proteins in different species showed that goat was closely related to sheep, domestic cattle and wild boar compared with other species, and the phylogenetic analysis was consistent with the results of zoological classification.
【作者单位】：河北农业大学动物科技学院;
【基金】：国家现代肉羊产业技术体系项目(CARS-39)
【分类号】：S827

【参考文献】