ERK信号转导通路对家兔脑炎原虫性脑肉芽肿形成的影响

发布时间：2018-06-28 05:36

本文选题：家兔脑炎原虫 + 脑肉芽肿　；参考：《中国兽医科学》2017年09期

【摘要】：为了探讨ERK信号转导通路对脑炎原虫性脑肉芽肿形成的影响,应用普通染色、特殊染色、免疫组织化学技术和Western-blot技术分别对脑炎原虫性脑肉芽肿进行观察,分析脑炎原虫性脑肉芽肿形成与信号转导通路中神经生长因子(NGF)、酪氨酸蛋白激酶(TPK)、丝裂源活化蛋白激酶(MAPK)和细胞外信号调节激酶(ERK)等四种关键性蛋白的关系。结果显示,所有患病家兔的脑组织中均有数量不等的脑肉芽肿和非化脓性脑炎变化;免疫组化染色显示,NGF、TPK、MAPK和ERK的表达在肉芽肿中的星形胶质细胞、血管内皮细胞和成纤维细胞中明显增高,呈强阳性反应,染成深棕色;用Western-blot检测,患病家兔脑组织中的NGF、TPK、MAPK和ERK等信号转导通路关键蛋白表丰度明显高于健康家兔。以上结果表明,ERK信号转导通路通过强大的级联反应参与了家兔脑炎原虫性脑肉芽肿的形成,在脑肉芽肿形成过程中促进星形胶质细胞、血管内皮细胞和成纤维细胞增殖。
[Abstract]:In order to investigate the effect of ERK signal transduction pathway on the formation of protozoan granuloma in encephalitis, general staining, special staining, immunohistochemical technique and Western-blot technique were used to observe the protozoan granuloma of the encephalitis, and the formation of brain granuloma and the nerve growth factor (NGF), casein in the signal transduction pathway of encephalitis protozoa were analyzed. The relationship between four key proteins such as acid protein kinase (TPK), mitogen activated protein kinase (MAPK) and extracellular signal regulated kinase (ERK). The results showed that all of the brain tissues of all the sick rabbits were different in brain granuloma and non suppurative encephalitis; immunohistochemical staining showed that the expression of NGF, TPK, MAPK and ERK in the granuloma The astrocytes, vascular endothelial cells and fibroblasts were significantly higher, and were strongly positive and dyed dark brown. The abundances of key proteins in the signal transduction pathways of NGF, TPK, MAPK and ERK in the brain tissues of the sick rabbits were significantly higher than those of healthy rabbits. The results showed that the ERK signal transduction pathway was stronger than that of the healthy rabbits. The cascade reaction participates in the formation of the protozoan granuloma of the rabbit encephalitis and promotes the proliferation of astrocytes, vascular endothelial cells and fibroblasts during the formation of brain granuloma.
【作者单位】：河南科技学院动物科技学院;西北农林科技大学动物医学院;
【基金】：国家自然科学基金面上项目(31372407)
【分类号】：S858.291

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