不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体和胰岛素受体表达的发育性变化

发布时间：2018-06-29 03:05

本文选题：猪 + 生理阶段　；参考：《动物营养学报》2017年02期

【摘要】：本试验旨在研究不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体(GLUT)1、GLUT4和胰岛素受体(IR)mRNA表达的发育规律。试验以1、7、30、60、90和150日龄蓝塘和长白两品种猪背最长肌、半膜肌和半腱肌组织样品c DNA为模板,采用实时荧光半定量PCR的方法,检测猪GLUT1和GLUT4 mRNA在不同骨骼肌中的表达模式。结果显示:1)1~150日龄蓝塘和长白猪背最长肌、半膜肌和半腱肌GLUT1 mRNA表达存在相似的发育变化规律,在1日龄时相对表达丰度最高。2)1~150日龄蓝塘和长白猪半膜肌和半腱肌GLUT4 mRNA相对表达丰度随日龄呈波形式变化,变化速度和程度存在品种差异。1日龄和150日龄时蓝塘猪背最长肌GLUT4mRNA相对表达丰度低于同日龄长白猪(P0.05),而在其余日龄时高于同日龄长白猪(P0.05)。3)蓝塘和长白猪背最长肌、半膜肌和半腱肌IR mRNA相对表达丰度在1和30日龄时表现出较高水平,而在7、60、90和150日龄时无显著差异(P0.05);同日龄蓝塘和长白猪之间IR mRNA相对表达丰度无显著性差异(P0.05)。结果表明:猪骨骼肌GLUT1 mRNA的表达在不同生理阶段存在差异,猪骨骼肌GLUT4 mRNA的表达在不同品种和生理阶段存在显著差异,且其与IR mRNA的表达相关性较弱,无明显规律。
[Abstract]:The aim of this study was to investigate the development of glucose transport vector (GLUT4) and insulin receptor (IR) mRNA expression in skeletal muscle of different breeds of pigs at different physiological stages. The expression patterns of porcine GLUT1 and GLUT4 mRNA in different skeletal muscles were detected by real-time fluorescence semi-quantitative PCR using cDNA of longissimus dorsi muscle, semimembranous muscle and semitendinosus muscle samples from Lantang and Changbai breeds at the age of 60 ~ 90 and 150 days respectively, and real-time fluorescence semi-quantitative PCR was used to detect the expression patterns of GLUT1 and GLUT4 mRNA in different skeletal muscles. The results showed that GLUT1 mRNA expression in the longissimus dorsi muscle, semimembranous muscle and semitendinosus muscle had similar developmental changes. The relative abundance of GLUT4 mRNA in the semimembranous muscle and semitendinosus of Landrace and Landrace pigs changed in wave form with the age of 1 day, and the relative abundance of GLUT4 mRNA in the semilemma muscle and semitendinosus of Landrace pig at the age of 1 day was the highest (.2). The relative expression abundance of GLUT4 mRNA in the longissimus dorsi muscle of Lantang pigs was lower than that of Landrace pigs of the same age (P0.05), but higher than that of Landrace and longissimus muscles of Landrace pigs at the other days of age (P0.05), and the expression of GLUT4 mRNA in the longissimus dorsi muscle of Landang pigs was higher than that of long-white pigs of the same age (P0.05). The relative abundance of IR mRNA in semimembranous muscle and semitendinosus muscle showed a higher level at 1 and 30 days of age, but there was no significant difference in IR mRNA relative expression abundance between Lantang and Landrace pigs at the age of 7 ~ 60,90 and 150 days (P0.05). The results showed that the expression of GLUT1 mRNA in pig skeletal muscle was different at different physiological stages, and the expression of GLUT4 mRNA in pig skeletal muscle was significantly different in different varieties and physiological stages, and the correlation between GLUT1 mRNA expression and IR mRNA expression was weak and had no obvious regularity.
【作者单位】：华南农业大学动物科学学院;
【基金】：国家重点基础研究发展计划项目(2012CB124701) 广东省科技计划项目(2016A020210104) 广州市科技计划项目(201510010258)
【分类号】：S828

【参考文献】