枯草芽孢杆菌J-4菌株产抑菌物质发酵条件优化

发布时间：2018-07-04 15:33

  本文选题：枯草芽孢杆菌 + 抑菌物质　； 参考：《河南农业科学》2017年02期

【摘要】：为了提高枯草芽孢杆菌J-4菌株发酵液中抑菌物质的含量,采用单因素试验和正交试验相结合的方法,对基础发酵培养基的组成(碳源、氮源、无机盐含量)和发酵条件(发酵时间、装液量、发酵温度、接种量)进行优化。优化后的培养基组成为葡萄糖1.00%、黄豆饼粉1.00%、MgSO_40.15%、Na_2HPO_40.10%、NaH_2PO_40.10%;发酵条件为发酵时间36 h、装液量100 mL(250 mL)、发酵温度37℃、摇瓶接种量5%。在此条件下,枯草芽孢杆菌J-4菌株抑菌圈面积提高50.25%。
[Abstract]:In order to increase the content of antimicrobial substances in fermentation broth of Bacillus subtilis J-4, the composition of basic fermentation medium (carbon source, nitrogen source, carbon source, nitrogen source) was studied by combining single factor test and orthogonal experiment. The content of inorganic salt and fermentation conditions (fermentation time, liquid loading, fermentation temperature, inoculation amount) were optimized. The optimized medium was composed of glucose 1.00, soybean cake powder 1.00 and MgSO40.15 + Na2HPO40.10 + Na2HPO40.100.The fermentation conditions were fermentation time 36 h, liquid loading 100 mL (250 mL), fermentation temperature 37 鈩，

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