CD44受体分子对口蹄疫病毒感染的影响

发布时间：2018-07-08 18:50

本文选题：CD + 口蹄疫病毒　；参考：《中国兽医科学》2016年12期

【摘要】：为探索CD44分子在口蹄疫病毒(FMDV)感染中的作用,揭示FMDV的感染机制,为开发阻断FMDV入侵的药物和高效疫苗提供新靶点。采用Western-blot、TCID50检测、激光共聚焦显微镜检测等方法,检测FMDV感染BHK21细胞后CD44的表达水平,以及用抑制剂MβCD抑制CD44表达后对FMDV增殖的影响。Western-blot结果显示,FMDV感染BHK21细胞后,CD44的表达量显著上升(P0.05);用MβCD抑制内源性CD44表达后,FMDV的增殖水平显著降低(P0.05)。TCID50检测结果显示,用MβCD抑制CD44表达后,FMDV的表达显著降低(P0.05)。激光共聚焦结果显示,FMDV感染BHK21细胞后CD44的表达量上升。表明,CD44分子参与FMDV的感染过程,可能作为调控分子直接或间接参与FMDV的入侵过程,仍需要进一步研究。
[Abstract]:In order to explore the role of CD44 molecules in FMDV infection, to reveal the mechanism of FMDV infection, and to provide a new target for the development of drugs and efficient vaccines to block FMDV invasion. The expression of CD44 in BHK21 cells infected with FMDV was detected by Western-blottTCID50 assay and confocal laser microscopy. The effect of inhibition of CD44 expression by M 尾 CD on the proliferation of FMDV. Western-blot results showed that the expression of CD44 was significantly increased after FMDV infection (P0.05), and the proliferation level of FMDV was significantly decreased after inhibiting the expression of endogenous CD44 by M 尾 CD (P0.05). The expression of FMDV was significantly decreased after the inhibition of CD44 expression by M 尾 CD (P 0.05). The results of confocal laser showed that the expression of CD44 in BHK21 cells was increased after FMDV infection. It is suggested that CD44 may be involved in the infection process of FMDV and may be directly or indirectly involved in the invasion process of FMDV.
【作者单位】：长江大学动物科学学院;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室;
【基金】：中国农业科学院“青年英才计划”择优支持项目 “十二五”国家科技支撑项目(2013BAD12B00) 国家国际科技合作专项(2014DFA31890) 国家自然科学基金项目(31672592)
【分类号】：S852.65

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