不同饱血状态微小牛蜱中肠和唾液菌群结构的分析

发布时间：2018-07-31 10:03

【摘要】：旨在探明微小牛蜱随吸血时间的延长,中肠和唾液微生物菌群结构的差异,以及优势菌属在中肠和唾液中增殖和迁移的特点。在无菌条件下采集半饱血、饱血微小牛蜱唾液和中肠内容物;提取细菌总DNA,PCR扩增其16SrDNA V3-V4区,Illumina MiSeq双端测序;对序列进行拼接和过滤、OTUs聚类、物种注释及多样性分析。结果显示,共得到134 317条序列,聚类后获得1 304个OTUs,半饱血雌成蜱唾液(SP)、饱血雌成蜱唾液(SF)、半饱血雌成蜱中肠内容物(MP)、饱血雌成蜱中肠内容物(MF)分别获得336、332、269、289个OTUs,其中152个OTUs为4个样本共有;4个样品在门水平上,以变形菌门和厚壁菌门为优势菌门,变形菌门在中肠中的含量大于唾液,而厚壁菌门反之;在属水平上,4个样本以柯克斯体属、不动杆菌属、甲基杆菌属、短波单胞菌属、立克次体属、埃希菌属为优势菌属,其中柯克斯体属、不动杆菌属在4个样本中含量均较大,柯克斯体属在半饱血状态下,含量高于饱血状态;不动杆菌属在唾液中的含量高于中肠。结果表明,微小牛蜱中肠和唾液中有复杂的微生物菌群结构;细菌在不同组织中的分布不同,其含量随吸血时间的延长而发生变化。
[Abstract]:The purpose of this study was to find out the differences of microflora structure between midgut and saliva with the prolongation of blood sucking time and the characteristics of proliferation and migration of dominant genera in midgut and saliva. The saliva and midgut contents were collected under aseptic condition, the 16SrDNA V3-V4 region of Illumina MiSeq was amplified by total bacterial DNA PCR, and the sequences were spliced and filtered by OTUs clustering, species annotation and diversity analysis. The results showed that 134,317 sequences were obtained. After clustering, 1 304 OTUs were obtained, saliva (SP), (SF), (MP), (MF), midgut contents of female adult ticks were obtained, respectively, of which 152 OTUs were found in 4 samples. The sample is at the door level, The content of Proteus in the midgut was higher than that in saliva, whereas the contrary was observed in the middle intestine. At the generic level, four samples were found in the genus Coxella, Acinetobacter, Methylbacillus, and short-wave monomonas. Among them, Coxella and Acinetobacter were all higher in the four samples, and the content of Coxella was higher than that of full blood in the condition of half full blood, and the genus Acinetobacter was the dominant genus in the genus Rickettsiella, and the genus Acinetobacter was the dominant genus in the genus Rickettsiella. Acinetobacter is higher in saliva than in midgut. The results showed that there were complex microbial flora structures in midgut and saliva of Bovine ticks, and the distribution of bacteria in different tissues varied with the prolongation of blood sucking time.
【作者单位】：湖南农业大学动物医学院;湖南畜禽安全生产协同创新中心;
【基金】：国家自然科学基金(31372431)
【分类号】：S852.746

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