2016年-2017年广东省部分地区猪繁殖与呼吸综合征病毒ORF5基因的遗传变异分析

发布时间：2018-08-12 19:33

【摘要】：为研究广东地区猪繁殖与呼吸综合征病毒(PRRSV)流行株ORF5基因的遗传演化情况,采用RT-PCR方法对2016年-2017年采自广东省地区猪场疑似PRRS猪肺脏组织样品中的PRRSV ORF5基因进行了扩增,并对这些毒株的核苷酸和氨基酸序列进行了生物信息学分析。结果表明,成功扩增出12株PRRSV流行毒株的ORF5基因片段。遗传演化分析表明,扩增出的毒株有10株与国内高致病性毒株亲缘关系较近,属于同一分支;2株与广东地区新报道的毒株QYYZ和GM2亲缘关系较近,属于同一分支。氨基酸序列比对结果表明,新分离的毒株在GP5抗原表位上存在广泛的变异。结果表明,该地区PRRSV毒株已经发生广泛变异。研究结果不仅揭示了广东地区流行的PRRSV ORF5基因的遗传演化特性,也为该地区PRRS的防控提供了科学依据。
[Abstract]:In order to study the genetic evolution of ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) epidemic strain in Guangdong, the PRRSV ORF5 gene was amplified by RT-PCR method from 2016 to 2017 in suspected PRRS pig lung tissue samples from pig farms in Guangdong province. The nucleotide and amino acid sequences of these strains were analyzed by bioinformatics. The results showed that the ORF5 gene fragments of 12 PRRSV strains were successfully amplified. Genetic evolution analysis showed that 10 of the amplified strains were closely related to the domestic high pathogenic virulence strains. Two strains belonging to the same branch were closely related to the newly reported strains of QYYZ and GM2 in Guangdong and belonged to the same branch. The results of amino acid sequence alignment showed that the newly isolated strains had extensive variation in GP5 epitopes. The results showed that the PRRSV strains in this area had been widely mutated. The results not only reveal the genetic and evolutionary characteristics of the prevalent PRRSV ORF5 gene in Guangdong, but also provide a scientific basis for the prevention and control of PRRS in this area.
【作者单位】：华南农业大学动物科学学院;信阳农林学院牧医工程学院;
【基金】：国家支撑计划项目(2015BAD12B02-5) 广州市科技计划项目(201508020062) 现代农业产业共性技术创新团队项目(2016LM2150)
【分类号】：S852.651

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