羊口疮病毒的分离鉴定及其遗传进化分析
发布时间：2018-08-17 18:49
【摘要】：为确定安徽肥东某山羊场发生的疑似羊口疮(ORF)的病原,利用MDBK细胞从送检的病羊唇部痂皮中分离获得病毒,通过PCR鉴定确定病原为羊口疮病毒(ORFV),并将其命名为ORFV/AH-FD/2016/China株。然后对分离株的B2L基因和F1L基因进行克隆、测序,并与其他ORFV序列进行遗传进化分析。结果表明:ORFV/AH-FD/2016/China株的B2L基因长1 137bp,编码279个氨基酸,F1L基因长1 023bp,编码341个氨基酸。与GenBank中收录的其他ORFV流行株相比,B2L基因的核苷酸同源性为96.9%~99.5%,推导的氨基酸同源性为95.5%~98.9%;F1L基因的核苷酸同源性为95.3%~98.3%,推导的氨基酸同源性为94.9%~99.1%。利用软件构建系统进化树,根据B2L基因遗传进化分析结果表明,该分离株与甘肃分离株的亲缘关系最近;根据F1L基因遗传进化分析结果表明,该分离株与福建分离株亲缘关系最近。基于B2L基因和F1L基因的遗传进化分析,其分离得到的病毒株与国内不同地区分离毒株的遗传差异不大。该研究为ORFV的后续研究提供了临床试验材料和流行病学依据。
[Abstract]:In order to determine the pathogen of suspected (ORF) in a goat farm in Feidong, Anhui Province, the virus was isolated from the scab skin of infected sheep lip by MDBK cells. The pathogen was identified as (ORFV), by PCR and named ORFV/AH-FD/2016/China strain. The B2L and F1L genes were cloned and sequenced, and the genetic evolution of B2L and F1L genes were analyzed with other ORFV sequences. The results showed that the B2L gene of the 10: ORFV / AH-FDR / 2016 / China strain was 1137 BP long, and the encoding 279 amino acid F1L gene was 1 023 BP long and 341 amino acids. The nucleotide homology of B2L gene and amino acid homology of F1L gene were 96.9and 99.5, respectively, and the deduced amino acid homology were 95.3398.3and 94.9m / 99.1, respectively, compared with the other ORFV epidemic strains included in GenBank. The nucleotide homology of B2L gene was 96.9% and 99.1% respectively, and the deduced amino acid homology was 95.9% (99.1%), while the nucleotide homology of F1L gene was 95.33.3%. The phylogenetic tree was constructed by software. According to the genetic evolution analysis of B2L gene, the genetic relationship between this isolate and Gansu isolate was the closest, and the result of F1L gene genetic evolution analysis showed that, The relationship between this isolate and Fujian isolate was close. Based on the genetic evolution analysis of B2L gene and F1L gene, the genetic difference between the virus strains isolated from B2L and F1L gene in different regions of China was not significant. This study provides clinical trial materials and epidemiological evidence for the follow-up study of ORFV.
【作者单位】： 安徽农业大学动物科技学院;宁波市疾病预防控制中心;
【基金】：国家自然科学基金资助项目(31602063) 安徽省自然科学基金资助项目(1508085QC60) 安徽农业大学稳定和引进人才科研项目(yj2015-16);安徽农业大学大学生科技创新基金项目(2016-2017)
【分类号】：S852.654
[Abstract]:In order to determine the pathogen of suspected (ORF) in a goat farm in Feidong, Anhui Province, the virus was isolated from the scab skin of infected sheep lip by MDBK cells. The pathogen was identified as (ORFV), by PCR and named ORFV/AH-FD/2016/China strain. The B2L and F1L genes were cloned and sequenced, and the genetic evolution of B2L and F1L genes were analyzed with other ORFV sequences. The results showed that the B2L gene of the 10: ORFV / AH-FDR / 2016 / China strain was 1137 BP long, and the encoding 279 amino acid F1L gene was 1 023 BP long and 341 amino acids. The nucleotide homology of B2L gene and amino acid homology of F1L gene were 96.9and 99.5, respectively, and the deduced amino acid homology were 95.3398.3and 94.9m / 99.1, respectively, compared with the other ORFV epidemic strains included in GenBank. The nucleotide homology of B2L gene was 96.9% and 99.1% respectively, and the deduced amino acid homology was 95.9% (99.1%), while the nucleotide homology of F1L gene was 95.33.3%. The phylogenetic tree was constructed by software. According to the genetic evolution analysis of B2L gene, the genetic relationship between this isolate and Gansu isolate was the closest, and the result of F1L gene genetic evolution analysis showed that, The relationship between this isolate and Fujian isolate was close. Based on the genetic evolution analysis of B2L gene and F1L gene, the genetic difference between the virus strains isolated from B2L and F1L gene in different regions of China was not significant. This study provides clinical trial materials and epidemiological evidence for the follow-up study of ORFV.
【作者单位】： 安徽农业大学动物科技学院;宁波市疾病预防控制中心;
【基金】：国家自然科学基金资助项目(31602063) 安徽省自然科学基金资助项目(1508085QC60) 安徽农业大学稳定和引进人才科研项目(yj2015-16);安徽农业大学大学生科技创新基金项目(2016-2017)
【分类号】：S852.654
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