灌注酵母培养物对绵羊血液生化指标、免疫及抗氧化功能的影响
发布时间：2018-08-22 15:26
【摘要】：本研究目的是对不同部位灌注不同剂量酵母培养物对绵羊血液生化指标、免疫及抗氧化功能影响进行比较研究,首先通过体外模拟瘤胃法筛选酵母培养物最适添加量;然后进行绵羊体内灌注试验,研究酵母培养物的作用效果和不同部位的效果差异;最后对灌注试验结果进行综合比较。选用9只体况良好、体重相近,装有瘤胃、十二指肠和回肠瘘管的小尾寒羊,随机分3组,每组3个重复,每个重复1只羊。I组为对照组,II组为体外最适宜添加量组,III组为体外第二适宜添加量组,在基础日粮条件下瘤胃、十二指肠、回肠分期灌注占日粮干物质0.0%、1.5%和2.0%的酵母培养物。其中瘤胃灌注试验预饲期14d,正式期21d,十二指肠和回肠灌注试验预饲期14d,正式期14d。论文共分为5个部分:1:体外模拟瘤胃法筛选酵母培养物的最适添加量。以试验日粮作为培养底物,并分别添加占培养底物干物质的0.0%、1.0%、1.5%、2.0%的酵母培养物。结果表明,1.5%添加量效果最好,2.0%添加量次之。其中各组pH只有12h出现显著差异,随着酵母培养物添加量的增加有下降趋势,2.0%组最低(P0.05);各组NH3-N在不同时间影响程度不同:6h时1.5%和2.0%组较低,12h时1.0%和1.5%较低,24h时对照组和1.0%较低;从整体趋势看,12h和24h的VFA变化趋势相同,与6h存在差异。2:瘤胃灌注酵母培养物结果表明:酵母培养物能够显著提高绵羊血清TP、GLU水平(P0.05),显著降低血清TG和UREA含量(P0.05),ALP有升高的趋势;能够不同程度提高血清中T-AOC,SOD和GSH-Px水平,并减少MDA生成量;第7d和21d极显著提高了绵羊显著提高了血清IgG含量(P0.01),2.0%组IgA含量在灌注第21d时显著高于对照组(P0.05);显著提高了IL-2、IL-6含量(P0.05),IFN-γ含量达到极显著水平(P0.01)。3:十二指肠灌注酵母培养物结果表明:通过十二指肠灌注酵母培养物,显著提高绵羊血清GLU含量(P0.05)。提高了绵羊血清T-AOC和T-SOD含量,降低MDA含量(P0.05);能够提高IgA、IgG和IL-6的含量,显著提高IL-2、IFN-γ含量(P0.05)。4:回肠灌注酵母培养物结果表明:回灌注酵母培养物有提高ALB和GLU含量的趋势;未对绵羊抗氧化指标产生显著的影响;随着灌注天数增加有提高IL-6含量的趋势。5:综合比较结果表明:本试验酵母培养物在绵羊上应用有实际效果,能够改善血液指标,提高抗氧化能力,提高免疫能力;不同灌注剂量之间并且未出现明显的效果差异,本试验条件下瘤胃灌注酵母培养物即可达到最佳效果,不需要就行过瘤胃保护处理。
[Abstract]:The purpose of this study was to compare the effects of different doses of yeast culture on blood biochemical indexes, immunity and antioxidant function of sheep. Then the effects of yeast culture and different parts of the yeast culture were studied. Finally, the results of the perfusion test were compared synthetically. Nine small tail Han sheep with rumen, duodenum and ileal fistula were randomly divided into 3 groups. Group I was the control group, group II was the best dosage group in vitro, group III was the second optimal dosage group in vitro. Under the basic diet condition, the rumen, duodenum and ileum were infused with 0.01.5% and 2.0% of the dry matter in the diet by stages. Among them, rumen perfusion test was prefed for 14 days, formal for 21 days, duodenum and ileum for 14 days, and formal for 14 days. The thesis was divided into five parts: 1: the optimum addition of yeast culture was screened by rumen mimic method in vitro. The experimental diet was used as the culture substrate, and the yeast culture consisted of 0.010% of the dry matter of the culture substrate and 1.5% and 2.0% of the dry matter of the culture substrate respectively. The results showed that 1.5% addition had the best effect of 2.0% addition. Among them, the pH value of each group was significantly different only at 12 h, and the lowest was in 2.0% group with the increase of yeast culture addition (P0.05), and the NH3-N was lower in 1.5% and 2.0% groups than in the control group at 12 h and 1.5% at 12 h and 1.0% at 24 h, respectively, at different time and at 6 h after addition of yeast (P0.05), and the control group was 1.0% and 1.0% lower than the control group at 12 h and 1.5%, respectively. According to the whole trend, the change trend of VFA in 12 h and 24 h was the same, and there was a difference between them at 6 h. The results showed that yeast culture could significantly increase serum TPGLU level (P0.05) and decrease serum TG and UREA content (P0.05). It can increase the levels of T-AOC SOD and GSH-Px in serum and reduce the amount of MDA in different degree. On the 7th and 21st days, the serum IgG content was significantly increased in sheep (P0.01). The IgA content in 2.0% group was significantly higher than that in the control group on the 21st day after perfusion (P0.05), and the IL-2IL-6 content (P0.05) was significantly increased in sheep (P0.01). 3: duodenal perfusion yeast culture. The results showed that yeast culture was infused through duodenum. The serum GLU content of sheep was significantly increased (P0.05). The contents of T-AOC and T-SOD in serum of sheep were increased, the contents of MDA were decreased (P0.05), and the contents of IgA T-AOC and IL-6 were increased significantly (P0.05). 4. The results showed that the contents of ALB and GLU were increased in the culture of ileal perfusion yeast. There was no significant effect on the antioxidant index of sheep, and with the increase of perfusion days, there was a tendency to increase the content of IL-6. 5. The comprehensive comparison showed that the yeast culture in this experiment had practical effect on sheep, and could improve the blood index. The effect of yeast culture on rumen perfusion could reach the best effect under the condition of this experiment, without the need of rumen protection treatment, the antioxidation ability was improved and the immune ability was improved, and there was no obvious difference in the effect between the different perfusion doses and there was no obvious difference in the effect.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826.5
[Abstract]:The purpose of this study was to compare the effects of different doses of yeast culture on blood biochemical indexes, immunity and antioxidant function of sheep. Then the effects of yeast culture and different parts of the yeast culture were studied. Finally, the results of the perfusion test were compared synthetically. Nine small tail Han sheep with rumen, duodenum and ileal fistula were randomly divided into 3 groups. Group I was the control group, group II was the best dosage group in vitro, group III was the second optimal dosage group in vitro. Under the basic diet condition, the rumen, duodenum and ileum were infused with 0.01.5% and 2.0% of the dry matter in the diet by stages. Among them, rumen perfusion test was prefed for 14 days, formal for 21 days, duodenum and ileum for 14 days, and formal for 14 days. The thesis was divided into five parts: 1: the optimum addition of yeast culture was screened by rumen mimic method in vitro. The experimental diet was used as the culture substrate, and the yeast culture consisted of 0.010% of the dry matter of the culture substrate and 1.5% and 2.0% of the dry matter of the culture substrate respectively. The results showed that 1.5% addition had the best effect of 2.0% addition. Among them, the pH value of each group was significantly different only at 12 h, and the lowest was in 2.0% group with the increase of yeast culture addition (P0.05), and the NH3-N was lower in 1.5% and 2.0% groups than in the control group at 12 h and 1.5% at 12 h and 1.0% at 24 h, respectively, at different time and at 6 h after addition of yeast (P0.05), and the control group was 1.0% and 1.0% lower than the control group at 12 h and 1.5%, respectively. According to the whole trend, the change trend of VFA in 12 h and 24 h was the same, and there was a difference between them at 6 h. The results showed that yeast culture could significantly increase serum TPGLU level (P0.05) and decrease serum TG and UREA content (P0.05). It can increase the levels of T-AOC SOD and GSH-Px in serum and reduce the amount of MDA in different degree. On the 7th and 21st days, the serum IgG content was significantly increased in sheep (P0.01). The IgA content in 2.0% group was significantly higher than that in the control group on the 21st day after perfusion (P0.05), and the IL-2IL-6 content (P0.05) was significantly increased in sheep (P0.01). 3: duodenal perfusion yeast culture. The results showed that yeast culture was infused through duodenum. The serum GLU content of sheep was significantly increased (P0.05). The contents of T-AOC and T-SOD in serum of sheep were increased, the contents of MDA were decreased (P0.05), and the contents of IgA T-AOC and IL-6 were increased significantly (P0.05). 4. The results showed that the contents of ALB and GLU were increased in the culture of ileal perfusion yeast. There was no significant effect on the antioxidant index of sheep, and with the increase of perfusion days, there was a tendency to increase the content of IL-6. 5. The comprehensive comparison showed that the yeast culture in this experiment had practical effect on sheep, and could improve the blood index. The effect of yeast culture on rumen perfusion could reach the best effect under the condition of this experiment, without the need of rumen protection treatment, the antioxidation ability was improved and the immune ability was improved, and there was no obvious difference in the effect between the different perfusion doses and there was no obvious difference in the effect.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826.5
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相关期刊论文 前7条
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本文编号：2197520
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