猪附红细胞体小鼠感染模型建立条件的优化

发布时间：2018-09-05 06:03

【摘要】：为探究猪附红细胞体感染特性,进而优化猪附红细胞体小鼠感染模型建立的条件,本试验设计了不同小鼠处理方式感染试验(A试验)、不同病原形式感染试验(B试验)、冻存病原感染试验(C试验)和小鼠模型血液再感染小鼠试验(D试验)。通过对每个试验中各组小鼠血液感染情况、感染首现时间、临床症状和平均感染时间的综合评估,分析不同小鼠处理方式、不同病原形式、冻存病原及小鼠模型血液再感染是否对小鼠模型的建立有影响。通过PCR检测、电镜观察和特异基因片段序列测定,进一步鉴定小鼠模型感染的病原与猪附红细胞体是否一致。结果显示,A试验中,昆明小鼠切除脾脏组感染效果最优;B试验中,猪附红细胞体阳性血液样本组感染效果最优;C试验中,阳性血液未冻存组感染效果优于冻存组;D试验中,猪阳性血液样本组、小鼠模型阳性血液样本组无明显差异。PCR检测、电镜观察和特异基因片段序列测定结果显示,小鼠模型感染的病原与猪附红细胞体一致。结果表明,不同小鼠处理方式、不同病原形式和病原的冻存等条件对猪附红细胞体小鼠感染模型的感染效果均有影响,通过对比,以猪阳性血液感染切除脾脏的昆明小鼠的方式建立小鼠感染模型效果最优。
[Abstract]:In order to study the characteristics of porcine eperythrozoon infection and optimize the conditions for establishing the infection model of porcine eperythrozoon mice, Infection test (A test), pathogen infection test (B test), cryopreserved pathogen infection test (C test) and blood reinfection test (D test) were designed in this experiment. Through the comprehensive evaluation of the blood infection, the first time of infection, the clinical symptoms and the average infection time of each group of mice in each experiment, the different treatment methods and different pathogenic forms of the mice were analyzed. Whether cryopreservation pathogen and blood re-infection of mouse model have influence on the establishment of mouse model. By means of PCR, electron microscopy and sequence analysis of specific gene fragments, the pathogen of mouse model infection was further identified to be consistent with that of porcine eperythrozoon. The results showed that the infection effect of Kunming mouse splenectomy group was better than that of frozen blood sample group, and that of porcine Eperythrozoon positive blood sample group was better than that of frozen blood group. There was no significant difference between the porcine positive blood sample group and the mouse model positive blood sample group. The results of electron microscope observation and specific gene fragment sequencing showed that the pathogen of mouse model infection was the same as that of porcine eperythrozoon. The results showed that different treatments, different pathogenetic forms and frozen storage of pathogens had an effect on the infection of mice infected with Eperythrozoon porcine. The infection model of Kunming mice with porcine positive blood infection was established by excision of spleen.
【作者单位】：延边大学农学院;
【基金】：国家自然科学基金(31460657) 吉林省科技厅青年科研基金(20150520129JH)
【分类号】：S855

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