猪胸膜肺炎放线杆菌小鼠感染模型的建立及在体内移行分布的研究
发布时间：2018-09-08 15:11
【摘要】：猪传染性胸膜肺炎(Porcine Contagious Pleuropneumonia, PCP)是胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae,APP）引起的一种高度传染性、致死性猪呼吸道传染病。近年来，规模化猪场的保育猪多发，APP有15个血清型，我国主要有血清1型、血清3型、血清5型和7型，本病的致病机制尚不明确，为揭示病原感染后各组织脏器的病理变化及体内的移行分布规律，本实验拟建立猪传染性胸膜肺炎放线杆菌病的动物模型。 实验采用猪传染性胸膜肺炎放线杆菌7型菌株，通过人工途径感染实验动物，探索其对实验动物的易感性和最佳感染途径，并利用免疫组化的方法检测动物感染后，其在模型动物体内的移行分布规律。 实验结果表明APP-7型菌株对小鼠具有较强的致病性，对小鼠的半数致死量为6.7×107CFU，筛选出的最佳感染途径为腹腔注射。通过对小鼠感染后的临床症状、组织器官的病理变化和死亡率等情况观察，发现与猪临床感染本病的表现具有高度的相似性，确定小鼠适合作为猪传染性胸膜肺炎放线杆菌感染试验的理想动物模型。免疫组化结果显示，细菌感染小鼠2小时后，，小鼠肺脏中能检测到细菌存在，感染4小时后，脾脏能检测到细菌的存在，随着感染时间的延长在小鼠肝脏、肾脏、心脏等主要组织器官中相继监测到细菌的分布，从感染后小鼠体内各脏器中再分离的细菌与攻毒菌株一致。 本研究通过感染小鼠试验，成功建立了APP-7菌株的小鼠动物模型，探索在细菌感染小鼠的不同时间后，体内各主要组织器官细菌的分布规律，揭示了小鼠组织器官内细菌的分布情况与造成动物机体组织损伤之间的关系，为进一步研究猪传染性胸膜肺炎放线杆菌的致病机理，疫苗的研发以及本病的科学防治奠定坚实的理论基础。
[Abstract]:Porcine infectious pleuropneumonia (Porcine Contagious Pleuropneumonia, PCP) is a highly infectious and fatal respiratory infection caused by Actinobacillus pleuropneumoniae (Actinobacillus pleuropneumoniae,APP). In recent years, there are 15 serotypes of app in large scale pig farms. In China, serotype 1, serotype 3, serotype 5 and serotype 7 are the main serotypes. The pathogenesis of the disease is not clear. In order to reveal the pathological changes of tissues and organs and the translocation and distribution of organs after pathogen infection, an animal model of actinomycosis of porcine infectious pleuropneumoniae was established in this experiment. In the experiment, Actinobacillus pleuropneumoniae type 7 was used to infect experimental animals by artificial way, to explore the susceptibility and the best way of infection to the experimental animals, and to detect the infection of animals by immunohistochemical method. Its migration and distribution in model animals. The results showed that the strain of APP-7 had strong pathogenicity to mice, and the median lethal dose to mice was 6.7 脳 107 CFU. The best route of infection was intraperitoneal injection. By observing the clinical symptoms, pathological changes of tissues and organs and mortality after infection in mice, it was found that there was a high similarity between the clinical manifestations of the disease and that of pigs. To determine that mice are suitable as an ideal animal model for actinobacillus pleuropneumoniae infection test. The results of immunohistochemistry showed that the presence of bacteria could be detected in the lungs of mice 2 hours after bacterial infection, and 4 hours after infection, the presence of bacteria could be detected in the spleen of mice with the prolongation of infection time in the liver and kidney of mice. The distribution of bacteria was detected in the heart and other major tissues and organs, and the bacteria isolated from the organs of the infected mice were consistent with the strains that attacked the virus. In this study, the animal model of APP-7 strain was successfully established by the experiment of infected mice, and the distribution of bacteria in main tissues and organs of mice was explored after different time of bacterial infection. In order to further study the pathogenic mechanism of Actinobacillus pleuropneumoniae in swine, the relationship between the distribution of bacteria in tissues and organs of mice and the injury of animal tissues was revealed. The research and development of vaccine and the scientific prevention and cure of this disease lay a solid theoretical foundation.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28
本文编号：2230884
[Abstract]:Porcine infectious pleuropneumonia (Porcine Contagious Pleuropneumonia, PCP) is a highly infectious and fatal respiratory infection caused by Actinobacillus pleuropneumoniae (Actinobacillus pleuropneumoniae,APP). In recent years, there are 15 serotypes of app in large scale pig farms. In China, serotype 1, serotype 3, serotype 5 and serotype 7 are the main serotypes. The pathogenesis of the disease is not clear. In order to reveal the pathological changes of tissues and organs and the translocation and distribution of organs after pathogen infection, an animal model of actinomycosis of porcine infectious pleuropneumoniae was established in this experiment. In the experiment, Actinobacillus pleuropneumoniae type 7 was used to infect experimental animals by artificial way, to explore the susceptibility and the best way of infection to the experimental animals, and to detect the infection of animals by immunohistochemical method. Its migration and distribution in model animals. The results showed that the strain of APP-7 had strong pathogenicity to mice, and the median lethal dose to mice was 6.7 脳 107 CFU. The best route of infection was intraperitoneal injection. By observing the clinical symptoms, pathological changes of tissues and organs and mortality after infection in mice, it was found that there was a high similarity between the clinical manifestations of the disease and that of pigs. To determine that mice are suitable as an ideal animal model for actinobacillus pleuropneumoniae infection test. The results of immunohistochemistry showed that the presence of bacteria could be detected in the lungs of mice 2 hours after bacterial infection, and 4 hours after infection, the presence of bacteria could be detected in the spleen of mice with the prolongation of infection time in the liver and kidney of mice. The distribution of bacteria was detected in the heart and other major tissues and organs, and the bacteria isolated from the organs of the infected mice were consistent with the strains that attacked the virus. In this study, the animal model of APP-7 strain was successfully established by the experiment of infected mice, and the distribution of bacteria in main tissues and organs of mice was explored after different time of bacterial infection. In order to further study the pathogenic mechanism of Actinobacillus pleuropneumoniae in swine, the relationship between the distribution of bacteria in tissues and organs of mice and the injury of animal tissues was revealed. The research and development of vaccine and the scientific prevention and cure of this disease lay a solid theoretical foundation.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28
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